• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

植物基因组消除过程中的灾难性染色体重组。

Catastrophic chromosomal restructuring during genome elimination in plants.

作者信息

Tan Ek Han, Henry Isabelle M, Ravi Maruthachalam, Bradnam Keith R, Mandakova Terezie, Marimuthu Mohan Pa, Korf Ian, Lysak Martin A, Comai Luca, Chan Simon Wl

机构信息

Department of Plant Biology, University of California, Davis, Davis, United States.

Genome Center, University of California, Davis, Davis, United States.

出版信息

Elife. 2015 May 15;4:e06516. doi: 10.7554/eLife.06516.

DOI:10.7554/eLife.06516
PMID:25977984
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4461816/
Abstract

Genome instability is associated with mitotic errors and cancer. This phenomenon can lead to deleterious rearrangements, but also genetic novelty, and many questions regarding its genesis, fate and evolutionary role remain unanswered. Here, we describe extreme chromosomal restructuring during genome elimination, a process resulting from hybridization of Arabidopsis plants expressing different centromere histones H3. Shattered chromosomes are formed from the genome of the haploid inducer, consistent with genomic catastrophes affecting a single, laggard chromosome compartmentalized within a micronucleus. Analysis of breakpoint junctions implicates breaks followed by repair through non-homologous end joining (NHEJ) or stalled fork repair. Furthermore, mutation of required NHEJ factor DNA Ligase 4 results in enhanced haploid recovery. Lastly, heritability and stability of a rearranged chromosome suggest a potential for enduring genomic novelty. These findings provide a tractable, natural system towards investigating the causes and mechanisms of complex genomic rearrangements similar to those associated with several human disorders.

摘要

基因组不稳定与有丝分裂错误及癌症相关。这种现象可导致有害的重排，但也会产生遗传新奇性，许多关于其起源、命运及进化作用的问题仍未得到解答。在此，我们描述了基因组消除过程中的极端染色体重组，这一过程源于表达不同着丝粒组蛋白H3的拟南芥植物杂交。破碎的染色体由单倍体诱导系的基因组形成，这与影响单个落后染色体并被分隔在微核中的基因组灾难相一致。对断点连接的分析表明，断裂后通过非同源末端连接（NHEJ）或停滞叉修复进行修复。此外，必需的NHEJ因子DNA连接酶4的突变导致单倍体回收率提高。最后，重排染色体的遗传性和稳定性表明了持久基因组新奇性的潜力。这些发现为研究与几种人类疾病相关的复杂基因组重排的原因和机制提供了一个易于处理的自然系统。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/66d5758b784e/elife-06516-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/f90cc7ecee34/elife-06516-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/b07942dde16b/elife-06516-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/71b369a55aaf/elife-06516-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/ef00336cfb9a/elife-06516-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/5a2c5dd85dd9/elife-06516-fig2-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/e54cacfc3499/elife-06516-fig2-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/cb6e4d294d72/elife-06516-fig2-figsupp4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/fea1dd96651e/elife-06516-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/1c73b1b733a1/elife-06516-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/8a25402490e6/elife-06516-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/0354789ae385/elife-06516-fig4-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/a58b79226121/elife-06516-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/0f98adfc3643/elife-06516-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/7f941ec133cc/elife-06516-fig5-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/6461576887c0/elife-06516-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/66d5758b784e/elife-06516-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/f90cc7ecee34/elife-06516-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/b07942dde16b/elife-06516-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/71b369a55aaf/elife-06516-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/ef00336cfb9a/elife-06516-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/5a2c5dd85dd9/elife-06516-fig2-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/e54cacfc3499/elife-06516-fig2-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/cb6e4d294d72/elife-06516-fig2-figsupp4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/fea1dd96651e/elife-06516-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/1c73b1b733a1/elife-06516-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/8a25402490e6/elife-06516-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/0354789ae385/elife-06516-fig4-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/a58b79226121/elife-06516-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/0f98adfc3643/elife-06516-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/7f941ec133cc/elife-06516-fig5-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/6461576887c0/elife-06516-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9403/4461816/66d5758b784e/elife-06516-fig7.jpg

相似文献

1
Catastrophic chromosomal restructuring during genome elimination in plants.植物基因组消除过程中的灾难性染色体重组。
Elife. 2015 May 15;4:e06516. doi: 10.7554/eLife.06516.
2
Marked contribution of alternative end-joining to chromosome-translocation-formation by stochastically induced DNA double-strand-breaks in G2-phase human cells.在G2期人类细胞中，随机诱导的DNA双链断裂通过替代末端连接对染色体易位形成的显著贡献。
Mutat Res Genet Toxicol Environ Mutagen. 2015 Nov;793:2-8. doi: 10.1016/j.mrgentox.2015.07.002. Epub 2015 Jul 4.
3
Detection of Chromothripsis in Plants.植物中染色体碎裂的检测
Methods Mol Biol. 2018;1769:119-132. doi: 10.1007/978-1-4939-7780-2_8.
4
The Arabidopsis AtLIG4 gene is required for the repair of DNA damage, but not for the integration of Agrobacterium T-DNA.拟南芥AtLIG4基因是DNA损伤修复所必需的，但不是农杆菌T-DNA整合所必需的。
Nucleic Acids Res. 2003 Jul 15;31(14):4247-55. doi: 10.1093/nar/gkg458.
5
Telomere dynamics and fusion of critically shortened telomeres in plants lacking DNA ligase IV.缺乏DNA连接酶IV的植物中极短端粒的端粒动态变化与融合
Nucleic Acids Res. 2007;35(19):6490-500. doi: 10.1093/nar/gkm472. Epub 2007 Sep 25.
6
Capture of extranuclear DNA at fission yeast double-strand breaks.裂殖酵母双链断裂处的核外DNA捕获
Genetics. 2005 Dec;171(4):1535-48. doi: 10.1534/genetics.105.046144. Epub 2005 Sep 2.
7
Breast cancer risk associated with genotypic polymorphism of the nonhomologous end-joining genes: a multigenic study on cancer susceptibility.非同源末端连接基因的基因型多态性与乳腺癌风险：一项关于癌症易感性的多基因研究。
Cancer Res. 2003 May 15;63(10):2440-6.
8
Mechanisms of DNA double strand break repair and chromosome aberration formation.DNA双链断裂修复及染色体畸变形成的机制。
Cytogenet Genome Res. 2004;104(1-4):14-20. doi: 10.1159/000077461.
9
Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.基于CRISPR/Cas的核酸酶和切口酶均可有效地用于拟南芥的基因组工程。
Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17.
10
Radiation-induced genomic rearrangements formed by nonhomologous end-joining of DNA double-strand breaks.由DNA双链断裂的非同源末端连接形成的辐射诱导基因组重排。
Cancer Res. 2001 May 15;61(10):3886-93.

引用本文的文献

1
Chromoanagenesis and Beyond: Catastrophic Events Shaping the Genome.染色体骤变及其他：塑造基因组的灾难性事件
Methods Mol Biol. 2025;2968:65-73. doi: 10.1007/978-1-0716-4750-9_4.
2
Chromothripsis.染色体碎裂
Methods Mol Biol. 2025;2968:3-33. doi: 10.1007/978-1-0716-4750-9_1.
3
Sterility and structural variation in an arabidopsis pedigree carrying a ring minichromosome.携带环状小染色体的拟南芥谱系中的不育性和结构变异

本文引用的文献

1
Naturally occurring differences in CENH3 affect chromosome segregation in zygotic mitosis of hybrids.CENH3中自然存在的差异会影响杂种合子有丝分裂中的染色体分离。
PLoS Genet. 2015 Jan 26;11(1):e1004970. doi: 10.1371/journal.pgen.1004970. eCollection 2015 Jan.
2
A haploid genetics toolbox for Arabidopsis thaliana.拟南芥的单倍体遗传学工具包。
Nat Commun. 2014 Oct 31;5:5334. doi: 10.1038/ncomms6334.
3
The Functional Topography of the Arabidopsis Genome Is Organized in a Reduced Number of Linear Motifs of Chromatin States.
Chromosome Res. 2025 Aug 1;33(1):16. doi: 10.1007/s10577-025-09776-0.
4
Exploring potential strategies for haploid induction based on double fertilization in plants.探索基于植物双受精的单倍体诱导潜在策略。
Plant Biotechnol J. 2025 Sep;23(9):4000-4016. doi: 10.1111/pbi.70197. Epub 2025 Jun 18.
5
Cytogenetic impact of gamma radiation and its effects on growth, yield and drought tolerance of maize (Zea mays L.).γ辐射对玉米（Zea mays L.）的细胞遗传学影响及其对生长、产量和耐旱性的作用。
BMC Plant Biol. 2025 Feb 3;25(1):141. doi: 10.1186/s12870-025-06111-x.
6
Centromere diversity and its evolutionary impacts on plant karyotypes and plant reproduction.着丝粒多样性及其对植物核型和植物繁殖的进化影响。
New Phytol. 2025 Mar;245(5):1879-1886. doi: 10.1111/nph.20376. Epub 2025 Jan 6.
7
Diverse Genome Structures among Eukaryotes May Have Arisen in Response to Genetic Conflict.真核生物多样的基因组结构可能是应对遗传冲突的结果。
Genome Biol Evol. 2024 Nov 1;16(11). doi: 10.1093/gbe/evae239.
8
Gradual chromosomal lagging drive programmed genome elimination in hemiclonal fishes from the genus Hypseleotris.渐发性染色体滞后驱动半克隆鱼类 Hypseleotris 属中程序性基因组消除。
Sci Rep. 2024 Nov 6;14(1):26866. doi: 10.1038/s41598-024-78278-6.
9
Wheat Cybrid Plants, OryzaWheat, Regenerated from Wheat-Rice Hybrid Zygotes via in Vitro Fertilization System Possess Wheat-Rice Hybrid Mitochondria.小麦-水稻杂种细胞系植株，由小麦-水稻杂种合子经体外受精再生而来，具有小麦-水稻杂种线粒体。
Plant Cell Physiol. 2024 Sep 3;65(8):1344-1357. doi: 10.1093/pcp/pcae074.
10
Non-homologous end joining shapes the genomic rearrangement landscape of chromothripsis from mitotic errors.非同源末端连接塑造了有丝分裂错误导致的染色体重排景观的脆性位点。
Nat Commun. 2024 Jul 4;15(1):5611. doi: 10.1038/s41467-024-49985-5.
拟南芥基因组的功能拓扑结构由数量减少的染色质状态线性基序组成。
Plant Cell. 2014 Jun;26(6):2351-2366. doi: 10.1105/tpc.114.124578. Epub 2014 Jun 16.
4
Unrepaired DNA damage facilitates elimination of uniparental chromosomes in interspecific hybrid cells.未修复的DNA损伤有助于消除种间杂交细胞中的单亲染色体。
Cell Cycle. 2014;13(8):1345-56. doi: 10.4161/cc.28296. Epub 2014 Mar 4.
5
Chromothripsis-like patterns are recurring but heterogeneously distributed features in a survey of 22,347 cancer genome screens.在对 22347 例癌症基因组筛查的调查中，类染色体重排模式是反复出现但具有异质性分布的特征。
BMC Genomics. 2014 Jan 29;15:82. doi: 10.1186/1471-2164-15-82.
6
Whole-genome sequencing identifies genomic heterogeneity at a nucleotide and chromosomal level in bladder cancer.全基因组测序在膀胱癌中鉴定出核苷酸和染色体水平的基因组异质性。
Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):E672-81. doi: 10.1073/pnas.1313580111. Epub 2014 Jan 27.
7
Chromothripsis and beyond: rapid genome evolution from complex chromosomal rearrangements.染色体重排与 Chromothripsis：复杂染色体重排导致的快速基因组演化
Genes Dev. 2013 Dec 1;27(23):2513-30. doi: 10.1101/gad.229559.113.
8
Catastrophic nuclear envelope collapse in cancer cell micronuclei.肿瘤细胞微核中灾难性的核膜崩溃。
Cell. 2013 Jul 3;154(1):47-60. doi: 10.1016/j.cell.2013.06.007.
9
Analysis of plant meiotic chromosomes by chromosome painting.利用染色体涂染技术分析植物减数分裂染色体
Methods Mol Biol. 2013;990:13-24. doi: 10.1007/978-1-62703-333-6_2.
10
PRICE: software for the targeted assembly of components of (Meta) genomic sequence data.PRICE：用于（元）基因组序列数据的组件靶向组装的软件。
G3 (Bethesda). 2013 May 20;3(5):865-80. doi: 10.1534/g3.113.005967.