Park Hye-Jin, Yang Hye Jeong, Kim Ki Hyun, Kim Sang Hee
Department of Food Science and Biotechnology, College of BioNano Technology Gachon University, Sungnam, Gyeonggi-do 461-701, Republic of Korea.
Division of Creative Food Science for Health, Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea.
J Ethnopharmacol. 2015 Jul 21;170:210-7. doi: 10.1016/j.jep.2015.04.012. Epub 2015 May 13.
Orostachys japonicus A. Berger (Crassulaceae) (OJ), well-known as Wa-song in Korea is a medicinal plant with immunoregulatory, anti-febrile, antidote, and anti-cancer activities. This study was aimed at evaluating the immunostimulatory effect of O. japonicus A. Berger and its possible mechanisms of action.
To evaluate the effect of OJ aqueous extract on macrophage activity, we evaluated the modulation of macrophage activation state by observing structural (phagocytic activities) and the production of nitric oxide increase. The effect of OJ aqueous extract on RAW264.7 cell viability were assessed using Cell Counting Kit (CCK)-8 assay. HPLC analysis was performed to identify potential active compounds of this extract.
The biological investigations indicated that OJ aqueous extract, among others, possessed the highest macrophage activation as indicated by NO production yield. The results showed that OJ aqueous extract exhibited antioxidant effects, which included scavenging activities against DPPH radicals. OJ aqueous extract increased the phagocytic activity of RAW 264.7 cells against IgG-opsonized red blood cells (RBC). The level of phosphorylated Syk kinase was increased in OJ aqueous extract-treated group as compared to control. Phosphorylation of PLC-γ was increased in the OJ aqueous extract-treated groups. Quercetin-3-O-rhamnose and kaempferol-3-O-rhamnose was detected in OJ aqueous extract by HPLC analysis.
OJ aqueous extract might play a pivotal ethnopharmacologic role as an immunostimulatory agent by promoting Fc gamma receptor (FcγR)-mediated phagocytosis of IgG-opsonized RBCs. On the basis of our results, OJ aqueous extract can enhance innate immunity and may serve as an adjuvant for tumor treatment.
日本瓦松（景天科）（OJ），在韩国被称为Wa-song，是一种具有免疫调节、解热、解毒和抗癌活性的药用植物。本研究旨在评估日本瓦松的免疫刺激作用及其可能的作用机制。
为了评估OJ水提取物对巨噬细胞活性的影响，我们通过观察结构（吞噬活性）和一氧化氮产生的增加来评估巨噬细胞激活状态的调节。使用细胞计数试剂盒（CCK）-8测定法评估OJ水提取物对RAW264.7细胞活力的影响。进行高效液相色谱（HPLC）分析以鉴定该提取物的潜在活性化合物。
生物学研究表明，OJ水提取物等具有最高的巨噬细胞激活作用，以一氧化氮产量表示。结果表明，OJ水提取物具有抗氧化作用，包括对DPPH自由基的清除活性。OJ水提取物增加了RAW 264.7细胞对IgG调理的红细胞（RBC）的吞噬活性。与对照组相比，OJ水提取物处理组中磷酸化Syk激酶的水平升高。OJ水提取物处理组中PLC-γ的磷酸化增加。通过HPLC分析在OJ水提取物中检测到槲皮素-3-O-鼠李糖和山奈酚-3-O-鼠李糖。
OJ水提取物可能通过促进Fcγ受体（FcγR）介导的IgG调理RBC的吞噬作用，作为免疫刺激剂发挥关键的民族药理学作用。根据我们的结果，OJ水提取物可以增强先天免疫力，并可作为肿瘤治疗的佐剂。
