INVIVO Co. Ltd., Iksan, Jeollabuk-do 54538, Republic of Korea.
Namwon Herb Agricultural Corp., Namwon, Jeollabuk-do 55770, Republic of Korea.
Biomed Res Int. 2019 Jan 6;2019:9461960. doi: 10.1155/2019/9461960. eCollection 2019.
In this study, we evaluated the immunity-enhancing effects of A. Berger (OJ). To examine the immune protective effect in vitro, primary mouse splenocytes were treated with water or ethanol extracts of OJ in the absence or presence of cyclophosphamide (CY), which is a cytotoxic, immunosuppressive agent. The extracts increased the propagation of splenocytes and inhibited CY-induced cytotoxicity. Further, to examine the immunostimulatory effects , adult Wistar rats were orally administered OJ extracts with or without CY treatment. With the administration of OJ extracts, CY-treated immunosuppressed rats showed improved physical endurance, as assessed by the forced swim test. In addition, extract administration increased not only the number of immunity-related cells but also the levels of plasma cytokines. OJ extracts also recovered splenic histology in CY-treated rats. These findings suggest that an OJ regimen can enhance immunity by increasing immune cell propagation and specific plasma cytokine levels.
在这项研究中，我们评估了 A. Berger（OJ）的增强免疫力的效果。为了在体外检查免疫保护作用，用 OJ 的水或乙醇提取物处理原代小鼠脾细胞，在存在或不存在环磷酰胺（CY）的情况下，CY 是一种细胞毒性、免疫抑制药物。提取物增加了脾细胞的增殖，并抑制了 CY 诱导的细胞毒性。此外，为了检查免疫刺激作用，用 OJ 提取物对成年 Wistar 大鼠进行口服给药，同时进行或不进行 CY 处理。用 OJ 提取物处理后，接受 CY 处理的免疫抑制大鼠在强迫游泳试验中表现出更好的身体耐力。此外，提取物的给药不仅增加了与免疫相关的细胞数量，而且还增加了血浆细胞因子的水平。OJ 提取物还恢复了 CY 处理大鼠的脾脏组织学。这些发现表明，OJ 方案可以通过增加免疫细胞增殖和特定的血浆细胞因子水平来增强免疫力。
