Department of Biomedical Laboratory Science, Inje University, Gimhae 621-749, Korea.
BMB Rep. 2011 Jun;44(6):399-404. doi: 10.5483/BMBRep.2011.44.6.399.
In this study, powder of Orostachys japonicus A. Berger (O. japonicus) was extracted with 95% ethyl alcohol and fractionated using a series of organic solvents, including n-hexane (hexane), dichloromethane (DCM), ethylacetate (EtOAc), n-butanol (BuOH), and water (H(2)O). We investigated the anti-inflammatory effects of these O. japonicus extracts on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Their effects on the expression of inflammatory mediators and transcription factors were analyzed by Western blotting. DCM fraction significantly inhibited formation of reactive oxygen species (ROS) as well as nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. Phosphorylation of the pro-inflammatory transcription factor complex nuclear factor-kappa B (NF-κB) p65 and expression of inducible nitric oxide synthase (iNOS), one of its downstream proteins, were also suppressed by DCM fraction. These effects were regulated by upsteam proteins in the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase/Akt (PI3K/Akt) signaling pathways. Taken together, our data suggest that O. japonicus could be used as a potential source for anti-inflammatory agents.
在这项研究中，采用 95%的乙醇提取朝鲜蓟（Orostachys japonicus A. Berger）粉末，并使用一系列有机溶剂进行分级，包括正己烷（hexane）、二氯甲烷（DCM）、乙酸乙酯（EtOAc）、正丁醇（BuOH）和水（H2O）。我们研究了这些朝鲜蓟提取物对脂多糖（LPS）刺激的 RAW 264.7 细胞的抗炎作用。通过 Western blot 分析了它们对炎症介质和转录因子表达的影响。DCM 部分显著抑制 LPS 刺激的 RAW 264.7 细胞中活性氧（ROS）和一氧化氮（NO）的形成。DCM 部分还抑制了促炎转录因子复合物核因子-κB（NF-κB）p65的磷酸化及其下游蛋白之一诱导型一氧化氮合酶（iNOS）的表达。这些作用受丝裂原激活蛋白激酶（MAPK）和磷酸肌醇 3-激酶/蛋白激酶 B（PI3K/Akt）信号通路中的上游蛋白调节。总之，我们的数据表明朝鲜蓟可作为抗炎药物的潜在来源。
