Suárez-Bonnet Alejandro, Herráez Pedro, Aguirre Maria, Suárez-Bonnet Elena, Andrada Marisa, Rodríguez Francisco, Espinosa de Los Monteros Antonio
Unit of Histology and Animal Pathology, Institute for Animal Health, Veterinary School, University of Las Palmas de Gran Canaria, Arucas, Spain.
Unit of Histology and Animal Pathology, Institute for Animal Health, Veterinary School, University of Las Palmas de Gran Canaria, Arucas, Spain.
Urol Oncol. 2015 Jul;33(7):332.e1-7. doi: 10.1016/j.urolonc.2015.04.006. Epub 2015 May 13.
The study of the expression of 14-3-3σ, p53, and vimentin proteins in canine transitional cell carcinoma (TCC) evaluating differences with normal bladder tissues, and the association with clinicopathological variables.
We analyze by immunohistochemistry in 19 canine TCCs the expression of 14-3-3σ, p53, and vimentin using monoclonal antibodys. A semiquantitative scoring method was employed and statistical analysis was performed to display relationships between variables.
In contrast to normal urinary bladder epithelium, which showed high levels of 14-3-3σ, its expression was decreased in 53% of the studied tumors (P = 0.0344). The 14-3-3σ protein was expressed by neoplastic emboli and by highly infiltrative neoplastic cells. The p53 protein was expressed in 26% of TCCs, but no significant association between 14-3-3σ and p53 was detected. Neoplastic epithelial cells displayed vimentin immunoreactivity in 21% of TCCs, and a positive correlation with mitotic index was observed (P = 0.042). Coexpression of vimentin and 14-3-3σ by highly infiltrative neoplastic cells was also observed.
14-3-3σ is deregulated in canine TCCs and its expression by highly infiltrative tumor cells may be related to the acquisition of aggressive behavior. Furthermore, this article reinforce the role of canine TCC as relevant model of human urothelial carcinoma and we suggest 14-3-3σ as a potential therapeutic target. Further studies are necessary to clarify the role of 14-3-3σ in canine TCC.
研究14-3-3σ、p53和波形蛋白在犬移行细胞癌（TCC）中的表达，评估其与正常膀胱组织的差异以及与临床病理变量的相关性。
我们使用单克隆抗体通过免疫组织化学分析19例犬TCC中14-3-3σ、p53和波形蛋白的表达。采用半定量评分方法并进行统计分析以显示变量之间的关系。
与显示高水平14-3-3σ的正常膀胱上皮相反，在所研究的肿瘤中有53%其表达降低（P = 0.0344）。14-3-3σ蛋白由肿瘤栓子和高度浸润性肿瘤细胞表达。p53蛋白在26%的TCC中表达，但未检测到14-3-3σ与p53之间有显著相关性。肿瘤上皮细胞在21%的TCC中显示波形蛋白免疫反应性，并且观察到与有丝分裂指数呈正相关（P = 0.042）。还观察到高度浸润性肿瘤细胞同时表达波形蛋白和14-3-3σ。
14-3-3σ在犬TCC中表达失调，其由高度浸润性肿瘤细胞表达可能与侵袭性行为的获得有关。此外，本文强化了犬TCC作为人类尿路上皮癌相关模型的作用，并且我们建议将14-3-3σ作为潜在的治疗靶点。需要进一步研究以阐明1
