Santos M, Correia-Gomes C, Santos A, de Matos A, Dias-Pereira P, Lopes C
Department of Microscopy, Instituto Ciências Biomédicas Abel Salazar, University of Porto, Porto, Portugal.
Epidemiology Research Unit, Future Farming Systems, Scotland's Rural College, Edinburgh, UK.
J Comp Pathol. 2015 Jul;153(1):22-7. doi: 10.1016/j.jcpa.2015.04.005. Epub 2015 May 12.
Histological grading of canine mammary carcinomas (CMCs) has been performed using an adaptation of the human Nottingham method. The histological grade could be a prognostic factor in CMC; however, no data are available concerning interobserver variability in grading. In this study we analyzed the interobserver reproducibility between three observers when assigning individual parameter scores and grade to 46 CMCs. The influence of tumour size and vascular invasion and/or lymph node metastases on the odds of grading disagreement was also evaluated. The mean kappa values were 0.71, 0.51, 0.69 and 0.70 for tubule formation, nuclear pleomorphism, mitotic counts and grade, respectively. There was moderate to good agreement in scoring parameters and tumour grading, with nuclear pleomorphism being least reproducible. These findings are similar to those of human studies. The odds of grading disagreement increased with tumour size, but decreased with the presence of vascular invasion and/or lymph node metastases. Individual scoring differences were moderated by reaching a consensus between two observers.
犬乳腺肿瘤（CMCs）的组织学分级采用了改良的人类诺丁汉方法。组织学分级可能是CMCs的一个预后因素；然而，目前尚无关于分级过程中观察者间变异性的数据。在本研究中，我们分析了三位观察者在对46例CMCs进行个体参数评分和分级时的观察者间再现性。还评估了肿瘤大小、血管侵犯和/或淋巴结转移对分级不一致几率的影响。小管形成、核多形性、有丝分裂计数和分级的平均kappa值分别为0.71、0.51、0.69和0.70。在评分参数和肿瘤分级方面存在中度至良好的一致性，其中核多形性的再现性最差。这些发现与人类研究的结果相似。分级不一致的几率随肿瘤大小增加而升高，但随血管侵犯和/或淋巴结转移的存在而降低。通过两位观察者达成共识，个体评分差异得到了缓解。
