Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Int Immunopharmacol. 2015 Sep;28(1):288-94. doi: 10.1016/j.intimp.2015.04.054. Epub 2015 May 13.
This study was conducted to investigate the protective roles of soy isoflavone in weaned pigs challenged with lipopolysaccharide (LPS). A total of 72 weaned piglets (14 days of age) were randomly allotted into either 0 (control group) or 40 mg/kg soy isoflavone (ISO) supplementation group. On days 7 and 14, half of the pigs in each group were challenged with LPS. Soy isoflavone increased average daily gain (ADG) and average daily feed intake (ADFI) of piglets challenged with LPS at days 7-14 (P<0.05). The incidence of diarrhea and plasma concentrations of malondialdehyde (MDA) and endotoxin in piglets from LPS group were higher than those in control group (P<0.05). Soy isoflavone reduced the incidence of diarrhea and plasma concentrations of endotoxin in piglets challenged with LPS (P<0.05). LPS challenge decreased (P<0.05) mRNA abundances of β-defensin 2 (pBD-2), mucin (MUC-4), zona occludens 1 (ZO-1), and occludin in jejunal mucosa of piglets, and soy isoflavone upregulated (P<0.05) mRNA abundances of ZO-1 and occludin in jejunal mucosa of piglets challenged with LPS. The present results demonstrated that both p38 and TLR4 pathways in jejunal mucosa of piglets were activated by LPS challenge (P<0.05), and soy isoflavone reduced their activations (P<0.05). Collectively, our results suggested that supplementation of soy isoflavone could partly attenuate the barrier-damaged effects of LPS and improve the intestinal barrier function of weaned piglets, at least partially by inhibiting activations of p38 and TLR4 dependent pathways induced by LPS. This study provides a potential usage of soy isoflavone for alleviating intestinal barrier damages of neonates and piglets.
本研究旨在探讨大豆异黄酮对脂多糖（LPS）攻毒断奶仔猪的保护作用。将 72 头 14 日龄断奶仔猪随机分为 0（对照组）或 40mg/kg 大豆异黄酮（ISO）补充组。第 7 和 14 天，每组一半的猪接受 LPS 攻毒。大豆异黄酮增加了 LPS 攻毒 7-14 日龄仔猪的平均日增重（ADG）和平均日采食量（ADFI）（P<0.05）。LPS 组仔猪腹泻发生率和血浆丙二醛（MDA）和内毒素浓度高于对照组（P<0.05）。大豆异黄酮降低了 LPS 攻毒仔猪的腹泻发生率和血浆内毒素浓度（P<0.05）。LPS 攻毒降低了仔猪空肠黏膜β-防御素 2（pBD-2）、粘蛋白（MUC-4）、紧密连接蛋白 1（ZO-1）和闭合蛋白的 mRNA 丰度（P<0.05），大豆异黄酮上调了 LPS 攻毒仔猪空肠黏膜的 ZO-1 和闭合蛋白的 mRNA 丰度（P<0.05）。本研究结果表明，LPS 攻毒激活了仔猪空肠黏膜中的 p38 和 TLR4 通路（P<0.05），大豆异黄酮降低了它们的激活（P<0.05）。综上所述，我们的研究结果表明，大豆异黄酮补充可部分减轻 LPS 引起的肠道屏障损伤，改善断奶仔猪的肠道屏障功能，至少部分通过抑制 LPS 诱导的 p38 和 TLR4 依赖途径的激活。本研究为大豆异黄酮缓解新生和仔猪肠道屏障损伤提供了一种潜在的用途。
