Yu Jiayi, Zheng Changbing, Zheng Jie, Duan Geyan, Guo Qiuping, Zhang Peiwen, Wan Mengliao, Duan Yehui
CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China.
University of Chinese Academy of Sciences, Beijing 100039, China.
Antioxidants (Basel). 2022 Nov 9;11(11):2215. doi: 10.3390/antiox11112215.
This study aimed to investigate the effects of lipopolysaccharide (LPS)-induced chronic immune stress on intestinal morphology and function, immune system, oxidative status, and mitochondrial function in piglets. Fifty healthy Duroc × Landrace × Yorkshire piglets (21 ± 2 days old, barrow, 6.98 ± 0.14 kg body weight) were selected and randomly allotted to five groups, which were slaughtered at 0 (0 group), 1, 5, 9, and 15 d of LPS injection. The results showed that compared with the piglets without LPS injection, LPS injection significantly impaired the intestinal morphology and permeability at 1, 5, and 9 d, as manifested by the increased serum lactic acid and decreased ratio of villus height to crypt depth (p < 0.05). Moreover, intestinal inflammation and oxidative and mitochondrial injury were caused at 1 d, as manifested by upregulated IL-6 mRNA expression, increased malondialdehyde content, and impaired mitochondrial morphology (p < 0.05). However, these parameters were restored to levels identical to 0 group at 9~15 d, accompanied by significantly increased antioxidant capacity, enhanced protein expression of CD3+ and CD68+, and upregulated mRNA abundance of genes related to mitochondrial biogenesis and functions (p < 0.05). Collectively, these results suggest that the intestinal injury of piglets caused by chronic immune stress could be self-repaired.
本研究旨在探讨脂多糖（LPS）诱导的慢性免疫应激对仔猪肠道形态与功能、免疫系统、氧化状态及线粒体功能的影响。选取50头健康的杜洛克×长白×大白仔猪（21±2日龄，公猪，体重6.98±0.14 kg），随机分为五组，分别在LPS注射后的0（0组）、1、5、9和15天进行屠宰。结果表明，与未注射LPS的仔猪相比，LPS注射在第1、5和9天显著损害肠道形态和通透性，表现为血清乳酸升高和绒毛高度与隐窝深度比值降低（p<0.05）。此外，在第1天引起肠道炎症以及氧化和线粒体损伤，表现为IL-6 mRNA表达上调、丙二醛含量增加和线粒体形态受损（p<0.05）。然而，这些参数在第9至15天恢复到与0组相同的水平，同时抗氧化能力显著增强、CD3+和CD68+的蛋白表达增强以及与线粒体生物发生和功能相关基因的mRNA丰度上调（p<0.05）。总体而言，这些结果表明慢性免疫应激引起的仔猪肠道损伤能够自我修复。
