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应用抗生物素蛋白-生物素技术改善细胞在纳米纤维基质上的黏附。

Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices.

作者信息

Pan Jian-feng, Liu Ning-hua, Shu Lin-yuan, Sun Hui

机构信息

Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 YiShan Road, Shanghai, 200233, China.

Department of Emergency, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 YiShan Road, Shanghai, 200233, China.

出版信息

J Nanobiotechnology. 2015 May 16;13:37. doi: 10.1186/s12951-015-0096-2.

DOI:10.1186/s12951-015-0096-2
PMID:25980573
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4461904/
Abstract

BACKGROUND

Electrospinning is an easy and effective technique to produce submicron fibers possessing a range of attractive characteristics such as interconnected porous structures similar to natural ECM and good resilience to movement. Rapid and efficient cell attachment to nanofibrous matrices is a necessary prerequisite in tissue engineering. Thus, the aim of this study is to evaluate poly(ε-caprolactone-co-lactide)/Pluronic (PLCL/Pluronic) nanofibrous matrices with avidin-biotin technology for improving cell adhesion for the first time.

RESULTS

PLCL/Pluronic nanofibers had relatively homogeneous fibers and interconnected porous structures. Pluronic significantly modified the hydrophilicity of nanofibrous matrices and PLCL/Pluronic nanofibrous matrices had better performance on maintaining cell proliferation. Avidin-biotin technology had no negative effect on the hydrophilic property, mechanical property and cell proliferation. Meanwhile, the attachment and spreading of adipose-derived stem cells (ADSCs) onto PLCL/Pluronic nanofibrous matrices with avidin-biotin technology was promoted obviously.

CONCLUSIONS

PLCL/Pluronic nanofibrous matrices inheriting the excellent characteristics of both PLCL and Pluronic have the better cell adhesion ability through avidin-biotin technology, implying a promising application in skin care, tissue regeneration and other related area.

摘要

背景

静电纺丝是一种简单有效的技术，可用于生产具有一系列吸引人特性的亚微米纤维，如类似于天然细胞外基质的相互连接的多孔结构以及良好的抗运动弹性。细胞快速有效地附着于纳米纤维基质是组织工程的必要前提条件。因此，本研究的目的是首次评估采用抗生物素蛋白-生物素技术的聚（ε-己内酯-共-丙交酯）/普朗尼克（PLCL/普朗尼克）纳米纤维基质，以改善细胞黏附。

结果

PLCL/普朗尼克纳米纤维具有相对均匀的纤维和相互连接的多孔结构。普朗尼克显著改变了纳米纤维基质的亲水性，且PLCL/普朗尼克纳米纤维基质在维持细胞增殖方面表现更佳。抗生物素蛋白-生物素技术对亲水性、机械性能和细胞增殖没有负面影响。同时，采用抗生物素蛋白-生物素技术明显促进了脂肪干细胞（ADSCs）在PLCL/普朗尼克纳米纤维基质上的附着与铺展。

结论

继承了PLCL和普朗尼克二者优异特性的PLCL/普朗尼克纳米纤维基质通过抗生物素蛋白-生物素技术具有更好的细胞黏附能力，这意味着在皮肤护理、组织再生及其他相关领域具有广阔的应用前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/f7a5c74734ad/12951_2015_96_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/b4bec90f74c6/12951_2015_96_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/2b6628d77869/12951_2015_96_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/3ef45d4e6974/12951_2015_96_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/5f10e935d9c1/12951_2015_96_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/9628c3e7073a/12951_2015_96_Fig5_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/3f5a1c613e7e/12951_2015_96_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/a9a4789c22e0/12951_2015_96_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/6f7185b1b4e9/12951_2015_96_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/f7a5c74734ad/12951_2015_96_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/b4bec90f74c6/12951_2015_96_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/2b6628d77869/12951_2015_96_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/3ef45d4e6974/12951_2015_96_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/5f10e935d9c1/12951_2015_96_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/9628c3e7073a/12951_2015_96_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/8f242cafce89/12951_2015_96_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/3f5a1c613e7e/12951_2015_96_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/a9a4789c22e0/12951_2015_96_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/6f7185b1b4e9/12951_2015_96_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/4461904/f7a5c74734ad/12951_2015_96_Fig10_HTML.jpg

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