Zhuang Y H
Zhonghua Yi Xue Za Zhi. 1989 Jul;69(7):365-8, 26.
Flow cytometric (FCM) and microspectrophotometric (MSP) measurements of nuclear DNA content were made in 52 fresh gastric carcinoma specimens and in 30 gastric mucosal specimens from patients with chronic gastritis DNA aneuploidy was found in 60% (32/53) cases of gastric carcinoma, and it appeared more frequently in well-or moderately differentiated tubular adenocarcinoma (90%) than in undifferentiated and mucous cell carcinoma (23.6%) (P less than 0.01). No aneuploidy was found in benign gastric mucosal samples, but their proliferative cell fractions were significantly higher than those in normal control gastric mucosal samples (p less than 0.01). The comparison of the results measured with FCM and MSP showed a high correlation rate (r = 0.07, p less than 0.005). The advantages and limitations of both methods are discussed, and the authors think that the two can be regarded complementary to each other and may be used in combination for more precise cytochemical analysis.
对52例新鲜胃癌标本和30例慢性胃炎患者的胃黏膜标本进行了流式细胞术（FCM）和显微分光光度法（MSP）检测细胞核DNA含量。在53例胃癌病例中，60%（32/53）发现DNA非整倍体，且在高分化或中分化管状腺癌（90%）中比未分化和黏液细胞癌（23.6%）中更常见（P<0.01）。在良性胃黏膜样本中未发现非整倍体，但其增殖细胞分数显著高于正常对照胃黏膜样本（P<0.01）。FCM和MSP测量结果的比较显示出高相关率（r=0.07，P<0.005）。讨论了两种方法的优缺点，作者认为两者可视为互补，可联合使用以进行更精确的细胞化学分析。
