Nakano O, Fujimaki E, Yamashiki H, Fujino Y, Orii S, Sato K, Sato S, Sasaki K, Sugai T
First Dept. of Internal Medicine, Iwate Medical University School of Medicine.
Gan To Kagaku Ryoho. 1994 May;21 Suppl 1:62-6.
In order to investigate whether or not DNA ploidy was altered in intramucosal gastric carcinomas, nuclear DNA content of biopsy specimen was measured using flow cytometry in 38 intramucosal carcinomas. DNA aneuploidy was detected in 27 of 38 lesions (71.1%), and noted more frequently in differentiated carcinomas than in undifferentiated ones (83.0% vs. 20.0%, p < 0.01). There was no significant relationship between the frequency of DNA aneuploidy and macroscopical type or tumor size. DNA aneuploidy was even found in two of three minute carcinomas (5 mm or less in diameter). DNA indices showed 1.2 or lower values in 40% of the lesions with DNA aneuploidy. The average value of DNA index was significantly larger in depressed type than in elevated type (p < 0.01). In conclusion, DNA ploidy is altered in most differentiated intramucosal carcinomas. A high resolution method is essential for accurate determination of DNA ploidy in intramucosal carcinomas, especially elevated ones.
为了研究黏膜内胃癌的DNA倍性是否发生改变，采用流式细胞术对38例黏膜内癌活检标本的细胞核DNA含量进行了测定。38个病灶中有27个（71.1%）检测到DNA非整倍体，在高分化癌中比未分化癌中更常见（83.0%对20.0%，p<0.01）。DNA非整倍体的频率与大体类型或肿瘤大小之间无显著关系。在3个微小癌（直径5mm或更小）中有2个甚至发现了DNA非整倍体。在40%的DNA非整倍体病灶中，DNA指数显示值为1.2或更低。凹陷型的DNA指数平均值明显高于隆起型（p<0.01）。总之，大多数高分化黏膜内癌的DNA倍性发生改变。高分辨率方法对于准确测定黏膜内癌尤其是隆起型癌的DNA倍性至关重要。
