Bayram Süleyman, Ülger Yakup, Sümbül Ahmet Taner, Kaya Berrin Yalınbaş, Rencüzoğulları Ahmet, Genç Ahmet, Sevgiler Yusuf, Bozkurt Onur, Rencüzoğulları Eyyüp
Department of Nursing, Adıyaman School of Health, Adıyaman University, 02040, Adıyaman, Turkey.
Department of Gastroenterology, Education and Research Hospital, Adıyaman University, 02040, Adıyaman, Turkey.
Fam Cancer. 2015 Dec;14(4):561-7. doi: 10.1007/s10689-015-9813-0.
An aberrant up-regulation of HOX transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), is associated with human cancers including gastric cancer (GC) and worse clinicopathological features. A naturally occurring functional single nucleotide polymorphism (SNP) rs920,778 (C→T) in the intronic enhancer of HOTAIR gene has been demonstrated to affect HOTAIR expression and cancer susceptibility. To investigate the association of the HOTAIR rs920778 polymorphism on the risk of GC susceptibility in Turkish population, a hospital-based case-control study was carried out consisting of 104 GC and 209 healthy control subjects matched on age and gender. The genotype frequency of HOTAIR rs920778 polymorphism was determined by using TaqMan Real-Time Polymerase Chain Reaction. No statistically significant differences were found in the allele or genotype distributions of the HOTAIR rs920778 polymorphism among GC and healthy control subjects (P > 0.05). Our results demonstrate that the HOTAIR rs920778 polymorphism has not been in any major role in genetic susceptibility to gastric carcinogenesis, at least in the population studied here. Independent studies are needed to validate our findings in a larger series, as well as in patients of different ethnic origins.
HOX转录本反义基因间RNA（HOTAIR）是一种长链非编码RNA（lncRNA），其异常上调与包括胃癌（GC）在内的人类癌症及更差的临床病理特征相关。HOTAIR基因内含子增强子中一种自然存在的功能性单核苷酸多态性（SNP）rs920778（C→T）已被证明会影响HOTAIR表达和癌症易感性。为了研究HOTAIR rs920778多态性与土耳其人群GC易感性风险的关联，开展了一项基于医院的病例对照研究，该研究由104例GC患者和209例年龄及性别匹配的健康对照者组成。采用TaqMan实时聚合酶链反应确定HOTAIR rs920778多态性的基因型频率。在GC患者和健康对照者之间，HOTAIR rs920778多态性的等位基因或基因型分布未发现统计学显著差异（P>0.05）。我们的结果表明，至少在本研究人群中HOTAIR rs920778多态性在胃癌发生的遗传易感性中未起主要作用。需要开展独立研究以在更大样本系列以及不同种族背景的患者中验证我们的发现。
