Dong Xueyi, Sun Baocun, Zhao Xiulan, Liu Zhiyong, Gu Qiang, Zhang Danfang, Zhao Nan, Wang Jinjing, Chi Jiadong
Department of Pathology, Tianjin Medical University, Tianjin, 300070 China ; Department of Pathology, Tianjin General Hospital, Tianjin Medical University, Tianjin, 300052 China.
Department of Pathology, Tianjin Medical University, Tianjin, 300070 China ; Department of Pathology, Tianjin Cancer Hospital, Tianjin Medical University, Tianjin, 300060 China ; Department of Pathology, Tianjin General Hospital, Tianjin Medical University, Tianjin, 300052 China.
J Biol Res (Thessalon). 2014 May 13;21(1):4. doi: 10.1186/2241-5793-21-4. eCollection 2014 Dec.
Physiological vasculogenesis in embryonic tissues share some important features with pathological neoangiogenesis in tumors. Linearly Patterned Programmed Cell Necrosis (LPPCN) and Vasculogenic Mimicry (VM) have been reported in tumors. The term VM refers to the aggressive tumor cells with CD31-negative phenotype to form Periodic Αcid Schiff (PAS)-positive network, that mimics the pattern of embryonic vasculogenic networks. LPPCN had been observed in our laboratory, and served as a spatial infrastructure for VM and endothelium-dependent vessel formation. Studies have been shown that hypoxia-inducible factor-1α (HIF-1α) can induce tumor cells to form vessel-like tubes and express genes associated with VM. Therefore, an analogous investigation has been carried out to determine if these patterns existed in mouse embryonic vasculogenesis.
In this essay, the results demonstrated that the number of Linearly Patterned Cell Αpoptosis (LPCA), embryo Vasculogenic Μimicry (embryo VM), endothelium-dependent vessels, and relative-protein of HIF-1α expression all showed time-dependent tendencies on E5.5-E9.5 (p < 0.05). The proteins CD133, VEGF, Twist, E-cadherin, and Vimentin showed local plexus distribution on E6.5-E7.5 (p < 0.05).
LPCA and embryo VM existed in embryonic vasculogenesis. The relative protein of HIF-1α regulated the mouse embryonic vasculogenesis.
胚胎组织中的生理性血管生成与肿瘤中的病理性新生血管生成具有一些重要特征。肿瘤中已报道了线性模式程序性细胞坏死（LPPCN）和血管生成拟态（VM）。术语VM是指具有CD31阴性表型的侵袭性肿瘤细胞形成的过碘酸希夫（PAS）阳性网络，其模仿胚胎血管生成网络的模式。LPPCN已在我们实验室中观察到，并作为VM和内皮依赖性血管形成的空间基础结构。研究表明，缺氧诱导因子-1α（HIF-1α）可诱导肿瘤细胞形成血管样管并表达与VM相关的基因。因此，已进行了一项类似的研究，以确定这些模式是否存在于小鼠胚胎血管生成中。
在本文中，结果表明，线性模式细胞凋亡（LPCA）、胚胎血管生成拟态（胚胎VM）、内皮依赖性血管的数量以及HIF-1α表达的相对蛋白在E5.5-E9.5上均呈现时间依赖性趋势（p < 0.05）。蛋白质CD133、VEGF、Twist、E-钙黏蛋白和波形蛋白在E6.5-E7.5上呈现局部丛状分布（p < 0.05）。
LPCA和胚胎VM存在于胚胎血管生成中。HIF-1α的相对蛋白调节小鼠胚胎血管生成。
