Thomas Stephen J, Aldstadt Jared, Jarman Richard G, Buddhari Darunee, Yoon In-Kyu, Richardson Jason H, Ponlawat Alongkot, Iamsirithaworn Sopon, Scott Thomas W, Rothman Alan L, Gibbons Robert V, Lambrechts Louis, Endy Timothy P
Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland; Department of Virology, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Geography, University at Buffalo, Buffalo, New York; Department of Entomology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Bureau of Epidemiology, Department of Disease Control Sciences, Ministry of Public Health, Nonthaburi, Thailand; Department of Entomology, University of California, Davis, Davis, California; Institute for Immunology and Informatics, University of Rhode Island, Providence, Rhode Island; Insect-Virus Interactions Group, Department of Genomes and Genetics, Institut Pasteur, Centre National de la Recherche Scientifique, Paris, France; Department of Infectious Diseases, State University of New York, Syracuse, New York; Fogarty International Center, National Institutes of Health, Bethesda, Maryland
Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland; Department of Virology, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Geography, University at Buffalo, Buffalo, New York; Department of Entomology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Bureau of Epidemiology, Department of Disease Control Sciences, Ministry of Public Health, Nonthaburi, Thailand; Department of Entomology, University of California, Davis, Davis, California; Institute for Immunology and Informatics, University of Rhode Island, Providence, Rhode Island; Insect-Virus Interactions Group, Department of Genomes and Genetics, Institut Pasteur, Centre National de la Recherche Scientifique, Paris, France; Department of Infectious Diseases, State University of New York, Syracuse, New York; Fogarty International Center, National Institutes of Health, Bethesda, Maryland.
Am J Trop Med Hyg. 2015 Jul;93(1):24-32. doi: 10.4269/ajtmh.14-0242. Epub 2015 May 18.
Dengue is of public health importance in tropical and sub-tropical regions. Dengue virus (DENV) transmission dynamics was studied in Kamphaeng Phet Province, Thailand, using an enhanced spatiotemporal surveillance of 93 hospitalized subjects with confirmed dengue (initiates) and associated cluster individuals (associates) with entomologic sampling. A total of 438 associates were enrolled from 208 houses with household members with a history of fever, located within a 200-m radius of an initiate case. Of 409 associates, 86 (21%) had laboratory-confirmed DENV infection. A total of 63 (1.8%) of the 3,565 mosquitoes collected were dengue polymerase chain reaction positive (PCR+). There was a significant relationship between spatial proximity to the initiate case and likelihood of detecting DENV from associate cases and Aedes mosquitoes. The viral detection rate from human hosts and mosquito vectors in this study was higher than previously observed by the study team in the same geographic area using different methodologies. We propose that the sampling strategy used in this study could support surveillance of DENV transmission and vector interactions.
登革热在热带和亚热带地区具有公共卫生重要性。在泰国彭世洛府，通过对93名确诊登革热的住院患者（起始病例）及相关聚集个体（关联个体）进行强化时空监测，并结合昆虫学采样，研究了登革热病毒（DENV）的传播动态。从位于起始病例200米半径范围内、有发热病史家庭成员的208户家庭中，共招募了438名关联个体。在409名关联个体中，86人（21%）经实验室确诊为DENV感染。在采集的3565只蚊子中，共有63只（1.8%）登革热聚合酶链反应呈阳性（PCR+）。与起始病例的空间距离与从关联病例和伊蚊中检测到DENV的可能性之间存在显著关系。本研究中人类宿主和蚊媒的病毒检测率高于该研究团队此前在同一地理区域使用不同方法所观察到的结果。我们认为，本研究中使用的采样策略可支持对DENV传播和媒介相互作用的监测。
