Moradi Hamid Reza, Erfani Majd Naeem, Esmaeilzadeh Saleh, Fatemi Tabatabaei Sayed Reza
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran;
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Vet Res Forum. 2015 Winter;6(1):23-9. Epub 2015 Mar 15.
Benign prostatic hyperplasia (BPH) is a common disease in human that gradual overgrowth of the prostate gland leads to impinge on the urethra with impairment in urinary function. Numerous plants improve uncontrolled growth of the prostate gland and improve urinary tract symptoms associated with BPH. In this study, 25 healthy adult male Wistar rats were divided randomly in five groups: G1 (Control group) received ordinary feed without any treatment, G2 received 10 mg kg(-1) testosterone subcutaneously, G3 received 50 mg kg(-1) nettle root extract orally, G4 received 50 mg kg(-1) nettle root extract orally and 10 mg kg(-1) testosterone, G5 received 10 mg kg(-1) almond oil (Almond oil was used as testosterone solvent) subcutaneously. After six weeks, volume and weight of each lobe were measured and samples were taken. The 5 to 6 µm thickness sections were made using paraffin embedding method and stained by hematoxylin and eosin and periodic acid-Schiff. The results showed that prostate volume and ratio of prostate to body weight were increased significantly in the testosterone. Histological and histometrical results showed that dorsal and lateral type 1 and 2 lobes were not changed significantly but the ventral and anterior lobes have changed significantly. Over all, the nettle root could prevent from some of prostatic hyperplasia effects, so that percentage of folded alveoli in ventral lobe reduced insignificantly.
良性前列腺增生（BPH）是一种常见的人类疾病，前列腺的逐渐过度生长会压迫尿道，导致排尿功能受损。许多植物可改善前列腺的无节制生长，并改善与BPH相关的尿路症状。在本研究中，将25只健康成年雄性Wistar大鼠随机分为五组：G1（对照组）接受普通饲料，不进行任何处理；G2皮下注射10 mg kg(-1)睾酮；G3口服50 mg kg(-1)荨麻根提取物；G4口服50 mg kg(-1)荨麻根提取物并皮下注射10 mg kg(-1)睾酮；G5皮下注射10 mg kg(-1)杏仁油（杏仁油用作睾酮溶剂）。六周后，测量每个叶的体积和重量，并采集样本。使用石蜡包埋法制作5至6 µm厚的切片，并用苏木精和伊红以及过碘酸-希夫氏试剂染色。结果表明，睾酮组的前列腺体积和前列腺与体重之比显著增加。组织学和组织计量学结果表明，背侧和外侧的1型和2型叶无明显变化，但腹侧和前叶有显著变化。总体而言，荨麻根可以预防一些前列腺增生的影响，腹侧叶中折叠肺泡的百分比略有降低。
