Bai Bin, Wu Jun, Sheng Wen-Tao, Zhou Bo, Zhou Li-Jie, Zhuang Wen, Yao Dong-Ping, Deng Qi-Yun
State Key Laboratory of Hybrid Rice, Longping Branch of Graduate School, Central South University, Changsha 410013, China.
Hunan Hybrid Rice Research Center, Changsha 410125, China.
Int J Mol Sci. 2015 May 18;16(5):11398-416. doi: 10.3390/ijms160511398.
Rice is highly sensitive to cold stress during reproductive developmental stages, and little is known about the mechanisms of cold responses in rice anther. Using the HiSeq™ 2000 sequencing platform, the anther transcriptome of photo thermo sensitive genic male sterile lines (PTGMS) rice Y58S and P64S (Pei'ai64S) were analyzed at the fertility sensitive stage under cold stress. Approximately 243 million clean reads were obtained from four libraries and aligned against the oryza indica genome and 1497 and 5652 differentially expressed genes (DEGs) were identified in P64S and Y58S, respectively. Both gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were conducted for these DEGs. Functional classification of DEGs was also carried out. The DEGs common to both genotypes were mainly involved in signal transduction, metabolism, transport, and transcriptional regulation. Most of the DEGs were unique for each comparison group. We observed that there were more differentially expressed MYB (Myeloblastosis) and zinc finger family transcription factors and signal transduction components such as calmodulin/calcium dependent protein kinases in the Y58S comparison group. It was also found that ribosome-related DEGs may play key roles in cold stress signal transduction. These results presented here would be particularly useful for further studies on investigating the molecular mechanisms of rice responses to cold stress.
水稻在生殖发育阶段对冷胁迫高度敏感，而关于水稻花药中冷响应机制的了解却很少。利用HiSeq™ 2000测序平台，在冷胁迫下对光温敏雄性不育系（PTGMS）水稻Y58S和P64S（培矮64S）在育性敏感阶段的花药转录组进行了分析。从四个文库中获得了约2.43亿条clean reads，并与籼稻基因组进行比对，分别在P64S和Y58S中鉴定出1497个和5652个差异表达基因（DEG）。对这些DEG进行了基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析。还对DEG进行了功能分类。两种基因型共有的DEG主要参与信号转导、代谢、转运和转录调控。大多数DEG在每个比较组中都是独特的。我们观察到，在Y58S比较组中，有更多差异表达的MYB（髓细胞组织增生）和锌指家族转录因子以及信号转导成分，如钙调蛋白/钙依赖蛋白激酶。还发现核糖体相关的DEG可能在冷胁迫信号转导中起关键作用。本文给出的这些结果对于进一步研究水稻对冷胁迫响应的分子机制将特别有用。
