用于直接检测产青霉素酶淋病奈瑟菌的多靶点聚合酶链反应检测法，以加强淋球菌耐药性监测
Multitarget PCR Assay for Direct Detection of Penicillinase-Producing Neisseria gonorrhoeae for Enhanced Surveillance of Gonococcal Antimicrobial Resistance.
作者信息
Buckley Cameron, Trembizki Ella, Baird Robert W, Chen Marcus, Donovan Basil, Freeman Kevin, Goire Namraj, Guy Rebecca, Lahra Monica M, Regan David, Whiley David M
机构信息
Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, Queensland, Australia The University of Queensland, Brisbane, Queensland, Australia.
Microbiology Laboratory, Pathology Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia.
出版信息
J Clin Microbiol. 2015 Aug;53(8):2706-8. doi: 10.1128/JCM.00540-15. Epub 2015 May 20.
Abstract
A multitarget PCR was developed for the direct detection of penicillinase-producing Neisseria gonorrhoeae (PPNG). The assay was validated by testing 342 PPNG isolates and 415 clinical samples. The method is suitable for routine detection of PPNG strains. Its multitarget approach reduces the potential for false-negative results caused by sequence variations.
摘要
开发了一种多靶点聚合酶链反应（PCR）用于直接检测产青霉素酶淋病奈瑟菌（PPNG）。通过检测342株PPNG分离株和415份临床样本对该检测方法进行了验证。该方法适用于PPNG菌株的常规检测。其多靶点方法降低了由序列变异导致假阴性结果的可能性。
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