Sekler Israel
Ben Gurion University of the Negev, Israel.
Biochem Biophys Res Commun. 2015 Apr 24;460(1):50-2. doi: 10.1016/j.bbrc.2015.02.170.
It is now the 40th anniversary of the Journal of Molecular and Cellular Cardiology paper by Ernesto Carafoli and colleagues. This seminal study described for the first time mitochondrial Ca(2+) extrusion and its coupling to Na(+). This short review will describe the profound impact that this work had on mitochondrial signaling and the cross talk between the mitochondria, the ER, and the plasma membrane. It will further tell how the functional identification and in particular its unique cation selectivity to both Li(+) and Na(+) eventually contributed to the identification of the mitochondrial Na(+)/Ca(2+) exchanger gene NCLX many years later. The last part will describe how molecular tools derived from NCLX identification are used to study the novel physiological aspects of Ca(2+) signaling.
今年是埃内斯托·卡拉福里及其同事发表于《分子与细胞心脏病学杂志》的论文40周年。这项开创性研究首次描述了线粒体Ca(2+)外排及其与Na(+)的偶联。这篇简短综述将阐述这项工作对线粒体信号传导以及线粒体、内质网和质膜之间相互作用产生的深远影响。还将讲述功能鉴定，特别是其对Li(+)和Na(+)独特的阳离子选择性最终如何在多年后促成了线粒体Na(+)/Ca(2+)交换体基因NCLX的鉴定。最后一部分将描述源自NCLX鉴定的分子工具如何用于研究Ca(2+)信号传导的新生理方面。
