Li Huaifen, Ye Lingling, Wang Yanwei, Xie Chenggen
Key Laboratory of Biomimetic Sensor and Detecting Technology of Anhui Province, School of Materials and Chemical Engineering, West Anhui University, Lu'an, Anhui, 237012, China.
Mikrochim Acta. 2017 Dec 2;185(1):5. doi: 10.1007/s00604-017-2578-4.
This paper reports on an electrochemical sensing system for L-cysteine. It is based on the use of hollow cubic CuO particles that were prepared in two steps. First, the CuO/ polystyrene (PS) composites were prepared by a surface ion exchange strategy for in-situ reductive deposition on the surface of carboxy-capped PS particles. Thereafter, the PS particles were removed from the CuO/PS composites by treatment with tetrahydrofuran (THF). The resulting hollow cubic CuO particles were placed in a Nafion matrix on a glassy carbon electrode (GCE) which exhibits high surface area, good site accessibility and excellent electrocatalytic activity for L-cysteine. The cyclic voltammetric response of the modified GCE to L-cysteine is about 2.8-fold stronger than when using a GCE modified with pure CuO. The detection limit for L-cysteine is lower by about 1 order of magnitude, and the working voltage is rather low (-0.08 V vs. Ag/AgCl). An excellent electrochemical selectivity for L-cysteine over other amino acids was also achieved. The method was successfully applied to the determination of L-cysteine in pharmaceutical samples. Graphical abstract An electrochemical sensing system for the detection of L-cysteine in amino acid injections has been established by using the hollow cubic CuO particles as recognition element.
本文报道了一种用于检测L-半胱氨酸的电化学传感系统。该系统基于两步法制备的空心立方氧化铜颗粒。首先，通过表面离子交换策略制备CuO/聚苯乙烯（PS）复合材料，以便在羧基封端的PS颗粒表面进行原位还原沉积。此后，通过用四氢呋喃（THF）处理从CuO/PS复合材料中去除PS颗粒。将所得的空心立方氧化铜颗粒置于玻碳电极（GCE）上的Nafion基质中，该电极对L-半胱氨酸具有高表面积、良好的位点可及性和优异的电催化活性。修饰后的GCE对L-半胱氨酸的循环伏安响应比使用纯CuO修饰的GCE时强约2.8倍。L-半胱氨酸的检测限降低了约1个数量级，工作电压相当低（相对于Ag/AgCl为-0.08 V）。该方法还实现了对L-半胱氨酸相对于其他氨基酸的优异电化学选择性。该方法成功应用于药物样品中L-半胱氨酸的测定。图形摘要：通过使用空心立方氧化铜颗粒作为识别元件，建立了一种用于检测氨基酸注射液中L-半胱氨酸的电化学传感系统。
