Bacher Petra, Scheffold Alexander
Department of Cellular Immunology, Clinic for Rheumatology and Clinical Immunology, Charité - University Medicine Berlin, 10117 Berlin, Germany.
Department of Cellular Immunology, Clinic for Rheumatology and Clinical Immunology, Charité - University Medicine Berlin, 10117 Berlin, Germany; German Rheumatism Research Centre (DRFZ) Berlin, Leibniz Association, 10117 Berlin, Germany.
Curr Opin Pharmacol. 2015 Aug;23:17-24. doi: 10.1016/j.coph.2015.04.005. Epub 2015 May 22.
T cells orchestrate and execute immune responses against certain antigens recognized by their antigen receptor. They can acquire a highly divers set of functional properties, which provide the basis for immune protection, but also for immune-pathologies and thus represent highly specific diagnostic and therapeutic targets. New cytometric technologies now allow identification and precise characterization of human conventional and regulatory T cells against basically any antigen and even within naive donors. These provide the basis for thorough analyses of immune protection against infections and to tackle unmet challenges such as T cell responses involved in tolerance and/or directed against undefined or complex antigens, that is, in autoimmunity or allergy. Together with the parallel evolution of single cell multi-parameter approaches this has revolutionized the quantitative and qualitative characterization of human T cells, bearing important diagnostic, prognostic or therapeutic potential.
T细胞协调并执行针对其抗原受体所识别的特定抗原的免疫反应。它们能够获得一系列高度多样的功能特性，这些特性不仅为免疫保护提供了基础，也为免疫病理提供了基础，因此代表了高度特异性的诊断和治疗靶点。新的细胞计数技术现在能够针对基本上任何抗原，甚至在未接触过抗原的供体内，识别并精确表征人类传统T细胞和调节性T细胞。这些技术为全面分析针对感染的免疫保护以及应对未满足的挑战奠定了基础，例如涉及耐受性和/或针对未定义或复杂抗原（即自身免疫或过敏中）的T细胞反应。随着单细胞多参数方法的同步发展，这彻底改变了人类T细胞的定量和定性表征，具有重要的诊断、预后或治疗潜力。
