用于检测与肠胃炎相关病原体的多重聚合酶链反应检测
Multiplex polymerase chain reaction tests for detection of pathogens associated with gastroenteritis.
作者信息
Zhang Hongwei, Morrison Scott, Tang Yi-Wei
机构信息
Luminex Corporation, 12212 Technology Boulevard, Austin, TX 78727, USA.
Clinical Microbiology Service, Memorial Sloan-Kettering Cancer Center, Weill Cornell Medical College, Cornell University, 1275 York Avenue, S428, New York, NY 10065, USA.
出版信息
Clin Lab Med. 2015 Jun;35(2):461-86. doi: 10.1016/j.cll.2015.02.006. Epub 2015 Apr 4.
Abstract
A wide range of enteric pathogens can cause infectious gastroenteritis. Conventional diagnostic algorithms are time-consuming and often lack sensitivity and specificity. Advances in molecular technology have provided new clinical diagnostic tools. Multiplex polymerase chain reaction (PCR)-based testing has been used in gastroenterology diagnostics in recent years. This article presents a review of recent laboratory-developed multiplex PCR tests and current commercial multiplex gastrointestinal pathogen tests. It focuses on two commercial syndromic multiplex tests: Luminex xTAG Gastrointestinal Pathogen Panel and BioFire FilmArray gastrointestinal test. Multiplex PCR tests have shown superior sensitivity to conventional methods for detection of most pathogens.
摘要
多种肠道病原体可导致感染性肠胃炎。传统的诊断方法耗时较长，且往往缺乏敏感性和特异性。分子技术的进步提供了新的临床诊断工具。近年来，基于多重聚合酶链反应（PCR）的检测已用于胃肠病学诊断。本文综述了近期实验室研发的多重PCR检测方法以及当前的商用多重胃肠道病原体检测方法。重点介绍了两种商业化的症候群多重检测方法：Luminex xTAG胃肠道病原体检测板和BioFire FilmArray胃肠道检测。多重PCR检测在检测大多数病原体方面已显示出比传统方法更高的敏感性。
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