Heidary Maryam, Momtaz Hassan, Madani Mahboobeh
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Falavarjan, IR Iran.
Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, IR Iran.
Iran Red Crescent Med J. 2014 Apr;16(4):e12329. doi: 10.5812/ircmj.12329. Epub 2014 Apr 5.
Acute infectious diarrhea is one of the most important causes of morbidity and mortality worldwide.
The objective of this study was to characterize antimicrobial resistant diarrheagenic Escherichia coli strains isolated from diarrheic children in Tehran, IR Iran.
In total, 550 stool samples from diarrheic pediatric patients, aged less than 60 months, were collected and immediately transferred to the laboratory. Isolation and identification of E. coli strains was done using bacteriological methods. Antimicrobial susceptibility testing was performed using the disk diffusion technique. Multiplex PCR was used to detect aadA1, tetA, tetB, dfrA1, qnr, aac (3)-IV, sul1, blaSHV, CITM, cat1, and cmlA antibiotic resistance genes.
From the total of 550 fecal samples examined, 154 samples (28%) were positive for diarraheagenic E. coli. High rates of antibiotic resistance were seen against penicillin ﴾100%), ampicillin ﴾89.6%﴿ and tetracycline ﴾83.1%﴿. Resistance against ciprofloxacin was low ﴾28.6%﴿. The prevalence of different resistance genes in the studied strains varied from 96.10% for aadA1 gene to 40.25% for sul1 gene. The frequencies of aadA1, tetA, tetB, dfrA1, qnr, aac(3)-IV, sul1, blaSHV, CITM, cat1, and cmlA genes were 96.10%, 85.06%, 84.41%, 51.94%, 72.07%, 54.54%, 40.25%, 57.79%, 90.25%, 59.74% and 60.38%, respectively.
Our results indicated that antibiotic resistance is increasing in diarraheagenic E. coli strains in Iran. It is imperative to develop strategies for prevention and control of resistant organisms. Changes in patterns of resistance against commonly used antibiotics in Iran indicate that an applied surveillance system and introduction of guidelines for appropriate antibiotic prescription are necessary.
急性感染性腹泻是全球发病和死亡的重要原因之一。
本研究的目的是对从伊朗德黑兰腹泻儿童中分离出的产腹泻大肠埃希菌菌株的抗菌药物耐药性进行特征分析。
总共收集了550份年龄小于60个月的腹泻儿科患者的粪便样本，并立即送往实验室。采用细菌学方法进行大肠埃希菌菌株的分离和鉴定。采用纸片扩散法进行抗菌药物敏感性试验。采用多重PCR检测aadA1、tetA、tetB、dfrA1、qnr、aac(3)-IV、sul1、blaSHV、CITM、cat1和cmlA抗生素耐药基因。
在总共检测的550份粪便样本中，154份（28%）产腹泻大肠埃希菌呈阳性。观察到对青霉素（100%）、氨苄西林（89.6%）和四环素（83.1%）的抗生素耐药率很高。对环丙沙星的耐药率较低（28.6%）。所研究菌株中不同耐药基因的流行率从aadA1基因的96.10%到sul1基因的40.25%不等。aadA1、tetA、tetB、dfrA1、qnr、aac(3)-IV、sul1、blaSHV、CITM、cat1和cmlA基因的频率分别为96.10%、85.06%、84.41%、51.94%、72.07%、54.54%、40.25%、57.79%、90.25%、59.74%和60.38%。
我们的结果表明，伊朗产腹泻大肠埃希菌菌株的抗生素耐药性正在增加。制定耐药生物的预防和控制策略势在必行。伊朗常用抗生素耐药模式的变化表明，需要一个应用监测系统并引入适当抗生素处方指南。
