Biswas J S, Al-Ali A, Rajput P, Smith D, Goldenberg S D
Centre for Clinical Infection and Diagnostics Research, King's College, London and Guy's and St Thomas' NHS Foundation Trust, London, UK.
Eur J Clin Microbiol Infect Dis. 2014 Nov;33(11):2075-81. doi: 10.1007/s10096-014-2177-9. Epub 2014 Jun 17.
Culture-dependent detection of gastroenteric bacteria is labour-intensive and does not provide results in a clinically relevant time frame. Several commercially available multiplex molecular panels are now available which may be more sensitive and could potentially provide rapid results. We compared the diagnostic accuracy, turnaround time and ease of use of three such molecular panels: the RIDA®GENE Bacterial Stool and EHEC/EPEC Panels (R-Biopharm AG, Darmstadt, Germany), the FTD® Bacterial Gastroenteritis Panel (Fast Track Diagnostics, Junglinster, Luxembourg) and the BD MAX™ Enteric Bacterial Panel (Becton Dickinson GmbH, Heidelberg, Germany). The results from 116 retrospective selected and 318 prospective unselected stool samples were compared with conventional culture-based techniques using a gold standard for a positive test of either culture or agreement in two of the three molecular panels. For most targets, the molecular panels were more sensitive than culture, detecting an additional 13 cases that culture missed. The laboratory turnaround time was under 3 h for all molecular panels, compared with 66.5 h for culture. The BD MAX™ panel was the fastest, easiest to use and most flexible.
依赖培养法检测肠道细菌不仅 labor-intensive，而且无法在临床相关的时间范围内得出结果。现在有几种市面上可买到的多重分子检测板，它们可能更灵敏，并且有可能快速得出结果。我们比较了三种此类分子检测板的诊断准确性、周转时间和易用性：RIDA®GENE 细菌粪便和肠出血性大肠杆菌/肠致病性大肠杆菌检测板（R-Biopharm AG，德国达姆施塔特）、FTD® 细菌性肠胃炎检测板（Fast Track Diagnostics，卢森堡容格林斯特）和 BD MAX™ 肠道细菌检测板（Becton Dickinson GmbH，德国海德堡）。将 116 份回顾性选择的和 318 份前瞻性未选择的粪便样本的检测结果与基于传统培养的技术进行比较，阳性检测的金标准为培养结果阳性或在三个分子检测板中的两个检测板中检测结果一致。对于大多数目标，分子检测板比培养法更灵敏，检测出了培养法遗漏的另外 13 例病例。所有分子检测板的实验室周转时间均在 3 小时以内，而培养法的周转时间为 66.5 小时。BD MAX™ 检测板速度最快、最易于使用且最灵活。
