Bhatti Muhammad Zeeshan, Ali Amjad, Saeed Asma, Saeed Ahmad, Malik Salman Akbar
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan / Institute of Biomedical Sciences, School of Life Science, East China Normal University, Dongchuan Road, Shanghai, China.
Institute of Biomedical Sciences, School of Life Science, East China Normal University, Dongchuan Road, Shanghai, China.
Pak J Pharm Sci. 2015 May;28(3):945-9.
To investigate the antitumor activity, brine shrimp lethality assay, antibacterial and antifungal activity of Methanol Extract (ME), Water Extract (WE), Acetone Extract (AE), Chloroform Extract (CE), Methanol-Water Extract (MWE), Methanol-Acetone Extract (MAE), Methanol-Chloroform Extract (MCE) of Ranunculus arvensis (L.). Antitumor activity was evaluated with Agrobacterium tumefaciens (At10) induced potato disc assay. Cytotoxicity was evaluated with brine shrimp lethality assay. Antibacterial activity was evaluated with six bacterial strains including Escherichia coli, Enterobacter aerogenes, Bordetella bronchiseptica, Klebsiella pneumoniae, Micrococcus luteus and Streptococcus anginosus and antifungal screening was done against five fungal strains including Aspergillus niger, A. flavus, A. fumigates, Fusarium solani and Mucor species by using disc diffusion method. Best antitumor activity was obtained with ME and WE, having highest IC50 values 20.27 ± 1.62 and 93.01 ± 1.33μg/disc. Brine shrimp lethality assay showed LC50 values of AE, MAE and ME were obtained as 384.66 ± 9.42μg/ml, 724.11 ± 8.01μg/ml and 978.7 ±8.01 μg/ml respectively. WE of R. arvensis revealed weak antimicrobial result against the tested microorganisms. On the other hand, the antifungal activity of the plant extracts was found to be insignificant. These findings demonstrate that extracts of R. arvensis possesses significant antitumor activity. Further extensive study is necessary to assess the therapeutic potential of the plant.
为研究毛茛（Ranunculus arvensis (L.)）的甲醇提取物（ME）、水提取物（WE）、丙酮提取物（AE）、氯仿提取物（CE）、甲醇 - 水提取物（MWE）、甲醇 - 丙酮提取物（MAE）、甲醇 - 氯仿提取物（MCE）的抗肿瘤活性、卤虫致死率测定、抗菌和抗真菌活性。采用根癌农杆菌（At10）诱导的马铃薯圆盘试验评估抗肿瘤活性。用卤虫致死率测定评估细胞毒性。用包括大肠杆菌、产气肠杆菌、支气管败血波氏杆菌、肺炎克雷伯菌、藤黄微球菌和咽峡炎链球菌在内的六种细菌菌株评估抗菌活性，并通过纸片扩散法对包括黑曲霉、黄曲霉、烟曲霉、茄病镰刀菌和毛霉属在内的五种真菌菌株进行抗真菌筛选。ME和WE获得了最佳的抗肿瘤活性，其最高IC50值分别为20.27±1.62和93.01±1.33μg/圆盘。卤虫致死率测定显示AE、MAE和ME的LC50值分别为384.66±9.42μg/ml、724.11±8.01μg/ml和978.7±8.01μg/ml。毛茛的WE对测试微生物显示出较弱的抗菌结果。另一方面，发现该植物提取物的抗真菌活性不显著。这些发现表明毛茛提取物具有显著的抗肿瘤活性。有必要进行进一步广泛的研究以评估该植物的治疗潜力。
