Student Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Appl Biochem Biotechnol. 2010 Sep;162(1):127-33. doi: 10.1007/s12010-009-8737-2. Epub 2009 Aug 15.
Microbial resistance is a major drawback in chemotherapy of microbial or fungal infection disease. In this study, the antifungal activity of ethanol extract of a selected plant (Sarcococca saligna) has been investigated against clinical isolates of Aspergillus niger, Aspergillus treus, Aspergillus flavus, and Aspergillus fumigatus. Also, the enhancement of the antifungal activity of fluconazole by this extract was further evaluated against mentioned test strains. Conventional disk diffusion method was used to assay the antifungal activity of S. saligna ethanol extract in the absence and presence of fluconazole. The highest antifungal activity was observed against A. treus. The ethanol extract of S. saligna enhanced the antifungal activity of fluconazole against A. niger and A. treus and A. flavus. At the highest tested contents (4 mg/disk), 1.15-, 0.64-, and 2.47-fold increases in inhibition zone surface area were observed for A. niger, A. treus, and A. flavus, respectively. However, no enhancing effect was observed for this plant extract against Aspergillus fumigates at tested contents (0.5, 1, 2, 3, and 4 mg/disk). In a separate experiment, the general cytotoxicity of the ethanol extract of S. saligna was examined with brine shrimp assay. This plant extract showed low cytotoxicity against Artemia salina (LC(50) = 186 microg/ml).
微生物耐药性是治疗微生物或真菌感染疾病化疗的主要障碍。在这项研究中，研究了一种选定植物（Sarcococca saligna）的乙醇提取物对黑曲霉、烟曲霉、黄曲霉和烟曲霉临床分离株的抗真菌活性。此外，还进一步评估了该提取物对氟康唑的抗真菌活性的增强作用。采用常规的圆盘扩散法，在没有和存在氟康唑的情况下，检测 S. saligna 乙醇提取物的抗真菌活性。结果表明，S. saligna 乙醇提取物对烟曲霉的抗真菌活性最强。S. saligna 的乙醇提取物增强了氟康唑对黑曲霉、烟曲霉和黄曲霉的抗真菌活性。在最高测试浓度（4mg/disk）下，对黑曲霉、烟曲霉和黄曲霉的抑制区表面积分别增加了 1.15、0.64 和 2.47 倍。然而，在测试浓度（0.5、1、2、3 和 4mg/disk）下，该植物提取物对烟曲霉没有增强作用。在另一个实验中，用卤虫试验检测了 S. saligna 乙醇提取物的一般细胞毒性。该植物提取物对盐水虾 Artemia salina 的细胞毒性较低（LC50=186μg/ml）。
