Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, China.
College of Mathematics and Computer Science, Wuhan Textile University, Wuhan 430073, China.
Carbohydr Polym. 2015 Sep 5;128:179-87. doi: 10.1016/j.carbpol.2015.04.012. Epub 2015 Apr 20.
The periphery of hyperbranched polysaccharides has many end groups that can be functioned and used as sites to interact with their surroundings. A water-insoluble hyperbranched β-d-glucan, coded as TM3a, extracted from sclerotia of an edible fungus (Pleurotus tuber-regium), was fractionated and modified chemically to obtain carboxymethylated derivatives (CTM3a). The solution properties of the carboxymethylated polysaccharides were studied systematically in phosphate buffer saline at 37 °C. The results indicated that the carboxymethylated glucans still kept hyperbranched structure after carboxymethylation, and existed as a swollen sphere-like chain conformation. The introduction of carboxymethylated groups permitted the formation of hydrogels through crosslinking CTM3a and silk fibroin with carbodiimide chemistry. The resultant hydrogels with porous and interconnected structure displayed good mechanical and swelling properties. This work provides some valuable and fundamental information of the natural hyperbranched polysaccharide from mushroom for further application in biomedical devices and tissue engineering.
超支化多糖的外围有许多末端基团，可以作为与周围环境相互作用的位点进行功能化和利用。从食用真菌（美味牛肝菌）的菌核中提取的一种不溶于水的超支化 β-d-葡聚糖，编号为 TM3a，经过分馏和化学修饰得到羧甲基化衍生物（CTM3a）。在 37°C 的磷酸盐缓冲盐溶液中系统研究了羧甲基化多糖的溶液性质。结果表明，羧甲基化后的葡聚糖仍保持超支化结构，呈溶胀的球链构象。引入羧甲基化基团后，通过碳化二亚胺化学将 CTM3a 和丝素蛋白交联形成水凝胶。具有多孔和相互连接结构的水凝胶具有良好的机械性能和溶胀性能。这项工作为进一步在生物医学装置和组织工程中应用蘑菇中的天然超支化多糖提供了一些有价值的基本信息。
