Zhuang Deshu, Han Jialong, Bi Liangjia, Wang Yueping, Hao Yanru, Zhou Qi, Cao Wenwu
Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China.
Department of Stomatology, The First Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China.
Drug Des Devel Ther. 2015 May 6;9:2545-51. doi: 10.2147/DDDT.S82347. eCollection 2015.
The aim of this study was to perform a histological evaluation of sonodynamic therapy (SDT) of hematoporphyrin monomethyl ether (HMME) on artificially induced periodontal disease in rats.
Submerging ligatures were placed at the subgingival region of the first maxillary molar in rats. Eighty rats were randomly assigned into four groups: group 1 received no treatment; group 2 was subjected to 50 μg/mL HMME alone; group 3 was treated with low-intensity ultrasound alone (1 W/cm(2)); and group 4 was treated with 50 μg/mL HMME plus ultrasound irradiation (1 MHz, 30 minutes). Ten rats in each group were euthanized at 7 and 15 days, and periodontal tissue samples were taken for histological examination.
The animals treated by SDT showed less bone loss (P<0.05) at all experimental periods than the other three groups. No significant differences were found between the control and HMME groups (P>0.05).
Our results suggest that HMME-mediated SDT can effectively alleviate the periodontal tissue destruction in artificially induced periodontitis in rats. Hence, SDT may have good clinic potential as a noninvasive treatment of periodontal diseases.
本研究旨在对血卟啉单甲醚（HMME）声动力疗法（SDT）治疗大鼠人工诱导的牙周病进行组织学评估。
在大鼠上颌第一磨牙龈下区域放置龈下结扎丝。80只大鼠随机分为四组：第1组不进行治疗；第2组单独给予50μg/mL HMME；第3组单独接受低强度超声治疗（1W/cm²）；第4组给予50μg/mL HMME加超声照射（1MHz，30分钟）。每组10只大鼠在第7天和第15天安乐死，取牙周组织样本进行组织学检查。
在所有实验阶段，接受SDT治疗的动物骨丢失均少于其他三组（P<0.05）。对照组和HMME组之间未发现显著差异（P>0.05）。
我们的结果表明，HMME介导的SDT可有效减轻大鼠人工诱导的牙周炎中牙周组织的破坏。因此，SDT作为牙周病的无创治疗方法可能具有良好的临床潜力。
