Kim Min Hwan, Woo Sang-Keun, Kim Kwang Il, Lee Tae Sup, Kim Chan Wha, Kang Joo Hyun, Kim Byung Il, Lim Sang Moo, Lee Kyo Chul, Lee Yong Jin
Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences , Seoul 139-706, Republic of Korea ; School of Life Sciences and Biotechnology, Korea University , Seoul 136-701, Republic of Korea.
Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences , Seoul 139-706, Republic of Korea.
ACS Med Chem Lett. 2015 Apr 7;6(5):528-30. doi: 10.1021/acsmedchemlett.5b00021. eCollection 2015 May 14.
The purpose of this study was to evaluate (64)Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate ((64)Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with (64)Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.
本研究的目的是评估(64)铜标记的十六烷基-1,4,7,10-四氮杂环十二烷四乙酸苯甲酸酯((64)Cu-DOTA-HB)(1)作为用于干细胞成像的正电子发射断层扫描(PET)放射性示踪剂的性能。十六烷基-DOTA-苯甲酸酯(DOTA-HB)(2)被(64)铜高效标记(>99%),脂肪来源干细胞(ADSCs)的细胞标记效率超过50%。用1进行标记不会损害细胞活力。在PET成像中,对正常大鼠心脏中肌肉注射移植的1标记的ADSCs进行了18小时的监测。这些结果表明,1可作为一种有前景的放射性示踪剂用于监测移植的干细胞。