Luo Lichao, Wang Huashan, Fan Chao, Liu Sen, Cai Yu
Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore, 117604 Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore, 117604.
Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore, 117604.
J Cell Biol. 2015 May 25;209(4):595-608. doi: 10.1083/jcb.201409142.
Stem cell self-renewal versus differentiation is regulated by the niche, which provides localized molecules that favor self-renewal. In the Drosophila melanogaster female germline stem cell (GSC) niche, Decapentaplegic (Dpp), a fly transforming growth factor β molecule and well-established long-range morphogen, acts over one cell diameter to maintain the GSCs. Here, we show that Thickveins (Tkv; a type I receptor of Dpp) is highly expressed in stromal cells next to Dpp-producing cells and functions to remove excess Dpp outside the niche, thereby spatially restricting its activity. Interestingly, Tkv expression in these stromal cells is regulated by multiple Wnt ligands that are produced by the niche. Our data demonstrate a self-restraining mechanism by which the Drosophila ovarian GSC niche acts to define its own boundary.
干细胞的自我更新与分化受微环境调控，微环境提供有利于自我更新的局部分子。在黑腹果蝇雌性生殖系干细胞（GSC）微环境中，Decapentaplegic（Dpp），一种果蝇转化生长因子β分子且是公认的长程形态发生素，作用于一个细胞直径的范围来维持GSC。在此，我们表明Thickveins（Tkv；Dpp的I型受体）在紧邻产生Dpp的细胞的基质细胞中高度表达，并起到清除微环境外多余Dpp的作用，从而在空间上限制其活性。有趣的是，这些基质细胞中Tkv的表达受微环境产生的多种Wnt配体调控。我们的数据证明了一种自我限制机制，通过该机制果蝇卵巢GSC微环境界定了自身边界。
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