Mitrokhin V M, Mladenov M I, Kamkin A G
Department of Fundamental and Applied Physiology, Russian National Research Medical University, Ostrovitjanova 1, Moscow 117997, Russia.
Department of Fundamental and Applied Physiology, Russian National Research Medical University, Ostrovitjanova 1, Moscow 117997, Russia; Faculty of Natural Sciences and Mathematics, Institute of Biology, "Ss. Cyril and Methodius" University, P.O. Box 162, 1000 Skopje, Macedonia.
Immunobiology. 2015 Sep;220(9):1107-12. doi: 10.1016/j.imbio.2015.05.003. Epub 2015 May 11.
Using the micro-electrode technique we studied the effects of interleukin-6 on bio-electric activity of rat atrial tissue under normal conditions and after gradual stretching. It was shown that IL-6 caused increasing of the duration of the action potential at the levels of 25, 50, and 90% re-polarization. The hump-like depolarization at APD90 appeared 7-10 min after initial stretching and transformed into single extra-potentials after tension removing. Perfusion with IL-6 for more than 20 min led to the appearance of atrial fibrillation even with the application of slight tension. Close observation of the IL-6 induced mechanisms and stretch induced APD alteration, confirmed the existence of a tight link between examined cytokine and stretch induced mechanisms.
我们采用微电极技术，研究了白细胞介素-6在正常条件下以及逐渐拉伸后对大鼠心房组织生物电活动的影响。结果表明，白细胞介素-6导致动作电位在复极化25%、50%和90%水平时的时程增加。初始拉伸后7-10分钟，APD90处出现驼峰样去极化，去除张力后转变为单个额外电位。用白细胞介素-6灌注超过20分钟，即使施加轻微张力也会导致房颤的出现。对白细胞介素-6诱导机制和拉伸诱导的动作电位时程改变的密切观察，证实了所检测的细胞因子与拉伸诱导机制之间存在紧密联系。
