Yang Y Z, Hao T L, Qian M, Dai W X, Huangfu Y M, Zhang Z H
J Tongji Med Univ. 1989;9(3):143-7. doi: 10.1007/BF02908964.
In our experiment, lymphocyte membrane was labeled by DPH fluorescence probe. The rate of rotation of the probe can be measured from the value of fluorescence polarization (PDPH). With this method useful information could be provided about membrane fluidity of lymphocytes. It was found that the F value (unit of lipid fluidity of membrane) of leukemic lymphocytes was obviously higher than that of normal ones. Furthermore, the F value of cultured leukemic Ts lymphocytes was the highest. In contrast with normal spleen T-lymphocytes or mixed lymphocytes, the response of malignant lymphocytes to the stimulation with ConA or PHA was reflected in the decrease of PDPH value or the increase of F value. Unexpectedly, the F value of T-lymphocytes from "615" mouse not injected with tumour cells was also higher than that of the mixed. The possibility of using the membrane fluidity as a diagnostic criterion was also discussed.
在我们的实验中，用DPH荧光探针标记淋巴细胞膜。可根据荧光偏振值（PDPH）测量探针的旋转速率。用这种方法可以提供有关淋巴细胞膜流动性的有用信息。发现白血病淋巴细胞的F值（膜脂质流动性单位）明显高于正常淋巴细胞。此外，培养的白血病Ts淋巴细胞的F值最高。与正常脾T淋巴细胞或混合淋巴细胞相比，恶性淋巴细胞对ConA或PHA刺激的反应表现为PDPH值降低或F值升高。出乎意料的是，未注射肿瘤细胞的“615”小鼠的T淋巴细胞的F值也高于混合淋巴细胞。还讨论了将膜流动性用作诊断标准的可能性。
