Forslund Ola, Schwartz Stefan, Olofsson Katarina, Rydell Roland
Department of Laboratory Medicine, Medical Microbiology, Lund University, Malmö
Department of Laboratory Medicine, Lund University, Lund.
Laryngoscope. 2016 Jan;126(1):122-7. doi: 10.1002/lary.25357. Epub 2015 May 22.
OBJECTIVES/HYPOTHESIS: To determine viral load of human papillomavirus type 6 (HPV6), physical state of HPV6-DNA, and transcription level of HPV6 E7-mRNA in laryngeal papilloma and in adjacent healthy mucosa.
Case series.
A papilloma biopsy was collected from each of 25 adult patients with respiratory recurrent papillomatosis. From 14 of the 25 patients, we first collected a biopsy from healthy mucosa of the false vocal fold and another from the papilloma. Quantity of HPV6 and E7-mRNA was measured by polymerase chain reaction.
For the papilloma, the median load of HPV6 was 41 copies/cell, and the lowest amount was 5.4 copies/cell. Human papillomavirus type 6 was detected in 50% (7/14) of the healthy mucosa, with a median of 1.1 copies/cell, and the highest amount was 6.6 copies/cell. Overall, viral load was higher in papilloma than in healthy mucosa (P < 0.05). The average HPV6 E2/E7-DNA ratio was 1.3, indicating an episomal state. Human papillomavirus type 6-mRNA was detected in all HPV6-DNA-positive samples. The transcription median ratio of HPV6-mRNA/HPV6-DNA was 1.5 in papilloma and 3.8 in healthy mucosa.
The amount of HPV6-DNA was consistently higher in the papilloma than in healthy mucosa. The transcription level of HPV6 E7 mRNA was similar in the papilloma and in normal mucosa. We suggest that interfering with replication of HPV6 and suppression of HPV6 to fewer than five copies/cell may be curative.
目的/假设：确定喉乳头状瘤及相邻健康黏膜中6型人乳头瘤病毒（HPV6）的病毒载量、HPV6-DNA的物理状态以及HPV6 E7-mRNA的转录水平。
病例系列研究。
收集25例成人复发性呼吸道乳头状瘤患者的乳头状瘤活检样本。在这25例患者中，我们首先从14例患者的假声带健康黏膜处及乳头状瘤处分别采集活检样本。通过聚合酶链反应测量HPV6和E7-mRNA的量。
对于乳头状瘤，HPV6的中位病毒载量为41拷贝/细胞，最低量为5.4拷贝/细胞。在50%（7/14）的健康黏膜中检测到6型人乳头瘤病毒，中位值为1.1拷贝/细胞，最高量为6.6拷贝/细胞。总体而言，乳头状瘤中的病毒载量高于健康黏膜（P<0.05）。HPV6 E2/E7-DNA的平均比值为1.3，表明为游离状态。在所有HPV6-DNA阳性样本中均检测到6型人乳头瘤病毒mRNA。HPV6-mRNA/HPV6-DNA的转录中位比值在乳头状瘤中为1.5，在健康黏膜中为3.8。
乳头状瘤中HPV6-DNA的量始终高于健康黏膜。HPV6 E7 mRNA在乳头状瘤和正常黏膜中的转录水平相似。我们建议干扰HPV6的复制并将HPV6抑制至低于5拷贝/细胞可能具有治愈作用。
