Laustsen Christoffer, Hansen Esben Søvsø Szocska, Kjaergaard Uffe, Bertelsen Lotte Bonde, Ringgaard Steffen, Stødkilde-Jørgensen Hans
MR Research Centre, Aarhus University Hospital, Aarhus, Denmark.
Danish Diabetes Academy, Odense, Denmark.
Magn Reson Med. 2015 Aug;74(2):558-63. doi: 10.1002/mrm.25692. Epub 2015 May 27.
Our aim was to determine the quantitative reproducibility of metabolic breakdown products in the kidney following intravenous injection of hyperpolarized [1-(13)C]pyruvate and secondly to investigate the metabolic effect on the pyruvate metabolism of oral sucrose load using dissolution dynamic nuclear polarization. By this technique, metabolic alterations in several different metabolic related diseases and their metabolic treatment responses can be accessed.
In four healthy pigs the lactate-to-pyruvate, alanine-to-pyruvate and bicarbonate-to-pyruvate ratio was measured following administration of regular cola and consecutive injections of hyperpolarized [1-(13)C]pyruvate four times within an hour.
The overall lactate-to-pyruvate metabolic profile changed significantly over one hour following an acute sucrose load leading to a significant rise in blood glucose.
The reproducibility of hyperpolarized magnetic resonance spectroscopy in the healthy pig kidney demonstrated a repeatability of more than 94% for all metabolites and, furthermore, that the pyruvate to lactate conversion and the blood glucose level is elevated following endogastric sucrose administration.
我们的目的是确定静脉注射超极化[1-(13)C]丙酮酸后肾脏中代谢分解产物的定量可重复性，其次是利用溶解动态核极化研究口服蔗糖负荷对丙酮酸代谢的代谢影响。通过这项技术，可以了解几种不同代谢相关疾病的代谢改变及其代谢治疗反应。
在四只健康猪中，在给予常规可乐并在一小时内连续四次注射超极化[1-(13)C]丙酮酸后，测量乳酸与丙酮酸、丙氨酸与丙酮酸以及碳酸氢盐与丙酮酸的比率。
急性蔗糖负荷后一小时内，总体乳酸与丙酮酸代谢谱发生显著变化，导致血糖显著升高。
健康猪肾脏中超极化磁共振波谱的可重复性表明，所有代谢物的重复性超过94%，此外，胃内给予蔗糖后，丙酮酸向乳酸的转化以及血糖水平升高。
