肾脏的超极化碳 (C) MRI：实验方案。
Hyperpolarized Carbon (C) MRI of the Kidney: Experimental Protocol.
机构信息
The MR Centre, Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
Mallinckrodt Institute of Radiology, Washington University, St. Louis, MO, USA.
出版信息
Methods Mol Biol. 2021;2216:481-493. doi: 10.1007/978-1-0716-0978-1_29.
Alterations in renal metabolism are associated with both physiological and pathophysiologic events. The existing noninvasive analytic tools including medical imaging have limited capability for investigating these processes, which potentially limits current understanding of kidney disease and the precision of its clinical diagnosis. Hyperpolarized C MRI is a new medical imaging modality that can capture changes in the metabolic processing of certain rapidly metabolized substrates, as well as changes in kidney function. Here we describe experimental protocols for renal metabolic [1-C]pyruvate and functional C-urea imaging step-by-step. These methods and protocols are useful for investigating renal blood flow and function as well as the renal metabolic status of rodents in vivo under various experimental (patho)physiological conditions.This chapter is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers. This experimental protocol is complemented by two separate chapters describing the basic concept and data analysis.
肾脏代谢的改变与生理和病理生理事件有关。现有的非侵入性分析工具，包括医学成像，对这些过程的研究能力有限，这可能限制了对肾脏疾病的现有认识及其临床诊断的精确性。极化 C MRI 是一种新的医学成像方式，可以捕捉某些快速代谢底物代谢处理的变化，以及肾功能的变化。在这里，我们描述了肾脏代谢 [1-C]丙酮酸和功能 C-尿素成像的实验方案。这些方法和方案可用于研究各种实验（病理）生理条件下，啮齿动物体内的肾脏血流和功能以及肾脏代谢状态。本章基于 COST 行动 PARENCHIMA 的工作，这是一个由欧洲合作科学技术（COST）计划资助的社区驱动网络，旨在提高肾脏 MRI 生物标志物的可重复性和标准化。本实验方案由另外两章补充，描述了基本概念和数据分析。
Zotero 插件