Karami Zohreh, Emam-Djomeh Zahra, Mirzaee Habib Allah, Khomeiri Morteza, Mahoonak Alireza Sadeghi, Aydani Emad
Department of Food Science and Technology, University of Gorgan, Gorgan, Iran.
Transfer Phenomena Lab (TPL), Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, University of Tehran, 31587-11167 Karaj, Iran.
J Food Sci Technol. 2015 Jun;52(6):3242-53. doi: 10.1007/s13197-014-1384-9. Epub 2014 Apr 30.
In present study, response surface methodology was used to optimize extraction condition of phenolic compounds from licorice root by microwave application. Investigated factors were solvent (ethanol 80 %, methanol 80 % and water), liquid/solid ratio (10:1-25:1) and time (2-6 min). Experiments were designed according to the central composite rotatable design. The results showed that extraction conditions had significant effect on the extraction yield of phenolic compounds and antioxidant capacities. Optimal condition in microwave assisted method were ethanol 80 % as solvent, extraction time of 5-6 min and liquid/solid ratio of 12.7/1. Results were compared with those obtained by soxhlet extraction. In soxhlet extraction, Optimum conditions were extraction time of 6 h for ethanol 80 % as solvent. Value of phenolic compounds and extraction yield of licorice root in microwave assisted (MAE), and soxhlet were 47.47 mg/g and 16.38 %, 41.709 mg/g and 14.49 %, respectively. These results implied that MAE was more efficient extracting method than soxhlet.
在本研究中，采用响应面法优化微波辅助从甘草根中提取酚类化合物的条件。考察的因素有溶剂（80%乙醇、80%甲醇和水）、液固比（10:1 - 25:1）和时间（2 - 6分钟）。实验按照中心复合旋转设计进行。结果表明，提取条件对酚类化合物的提取率和抗氧化能力有显著影响。微波辅助法的最佳条件是以80%乙醇为溶剂，提取时间为5 - 6分钟，液固比为12.7/1。将结果与索氏提取法得到的结果进行比较。在索氏提取中，最佳条件是以80%乙醇为溶剂，提取时间为6小时。微波辅助提取（MAE）和索氏提取中甘草根的酚类化合物含量和提取率分别为47.47mg/g和16.38%、41.709mg/g和14.49%。这些结果表明，微波辅助提取法比索氏提取法更高效。
