微波辅助提取水果中的酚类化合物：优化与鉴定。
Microwave-Assisted Extraction of Phenolic Compounds from Fruit: Optimization and Identification.
机构信息
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangdong Engineering Technology Research Center of Nutrition Translation, Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China.
South China Sea Bioresource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-Sen University, Guangzhou 510006, China.
出版信息
Molecules. 2018 Sep 29;23(10):2498. doi: 10.3390/molecules23102498.
A microwave-assisted extraction (MAE) technology optimized by response surface methodology (RSM) was established to extract phenolic compounds from the fruit of . The effects of solvent composition, ratio of solvent to material, temperature, time and microwave power on phenol yield were evaluated in single-factor tests. The three parameters exerting main impacts on phenol yield were further optimized by RSM. Under optimal extraction conditions (31.33% ethanol, solvent/material ratio of 32.21 mL/g, 52.24 °C, 45 min and 500 W), the total phenolic content was 39.02 ± 0.73 mg gallic acid equivalent (GAE)/g dry weight (DW). This MAE method performed better in comparison with two conventional methods, those being maceration (25.79 ± 1.03 mg GAE/g DW) and Soxhlet extraction (18.40 ± 1.34 mg GAE/g DW), using lower process temperature, shorter irradiation time, and lower organic solvent consumption. In addition, five flavonoids (epicatechin gallate, epicatechin, rutin, pigallocatechin and quercetin) and two phenolic acids (protocatechuic acid and chlorogenic acid) in the extract were identified and quantified using UPLC-MS/MS.
采用响应面法（RSM）优化了微波辅助提取（MAE）技术，用于从果实中提取酚类化合物。通过单因素试验评估了溶剂组成、溶剂与物料比、温度、时间和微波功率对酚产量的影响。通过 RSM 进一步优化了对酚产量有主要影响的三个参数。在最佳提取条件（31.33%乙醇、溶剂/物料比 32.21 mL/g、52.24°C、45 分钟和 500 W）下，总酚含量为 39.02±0.73 mg 没食子酸当量（GAE）/g 干重（DW）。与两种常规方法（浸渍法（25.79±1.03 mg GAE/g DW）和索氏提取法（18.40±1.34 mg GAE/g DW）相比，该 MAE 方法在较低的工艺温度、较短的辐射时间和较低的有机溶剂消耗下表现更好。此外，采用 UPLC-MS/MS 鉴定并定量了提取物中的 5 种类黄酮（表儿茶素没食子酸酯、表儿茶素、芦丁、儿茶素和槲皮素）和 2 种酚酸（原儿茶酸和绿原酸）。
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