MOE Key Laboratory of High Performance Polymer Materials and Technology, Collaborative Innovation Center of Chemistry for Life Sciences, and Department of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing, 210093 (P. R. China).
Angew Chem Int Ed Engl. 2015 Jul 6;54(28):8094-9. doi: 10.1002/anie.201503067. Epub 2015 Jun 3.
We have developed a nanosensor for tracking cancer metastasis by noninvasive real-time whole-body optical imaging. The nanosensor is prepared by the formation of co-micelles from a poly(N-vinylpyrrolidone)-conjugated iridium(III) complex (Ir-PVP) and poly(ε-caprolactone)-b-poly(N-vinylpyrrolidone) (PCL-PVP). The near-infrared phosphorescence emission of the nanosensor could be selectively activated in the hypoxic microenvironment induced by cancer cells. The detection ability of the nanosensor was examined in cells and different animal models. After intravenous injection, the nanosensor can be effectively delivered to the lung and lymph node, and cancer cell metastasis through bloodstream or lymphatics can be quickly detected with high signal-to-background ratio by whole-body imaging and organ imaging. Moreover, the nanosensor exhibits good biocompatibility both in vitro and in vivo. The nanosensor is believed to be a powerful tool for the diagnosis of cancer metastasis.
我们研发了一种通过非侵入式实时全身光学成象来追踪癌症转移的纳米传感器。该纳米传感器是通过将聚（N-乙烯基吡咯烷酮）接枝铱（III）配合物（Ir-PVP）与聚（己内酯）-b-聚（N-乙烯基吡咯烷酮）（PCL-PVP）形成共胶束来制备的。纳米传感器的近红外磷光发射可以在癌细胞诱导的缺氧微环境中被选择性激活。我们在细胞和不同的动物模型中检测了纳米传感器的检测能力。静脉注射后，纳米传感器可以有效地递送到肺部和淋巴结，并且可以通过全身成像和器官成像以高信噪比快速检测通过血液或淋巴系统的癌细胞转移。此外，纳米传感器在体外和体内均表现出良好的生物相容性。该纳米传感器有望成为癌症转移诊断的有力工具。
