Hamad Rania, Jayakumar Calpurnia, Ranganathan Punithavathi, Mohamed Riyaz, El-Hamamy Mahmoud M I, Dessouki Amina A, Ibrahim Abdelazim, Ramesh Ganesan
Department of Medicine and Vascular Biology Center, Georgia Regents University, Augusta, GA, USA.
Department of Pathology, Suez Canal University, Suez Canal, Egypt.
Clin Exp Pharmacol Physiol. 2015 Aug;42(8):843-8. doi: 10.1111/1440-1681.12433.
Cisplatin is a highly effective chemotherapeutic drug used to treat a wide variety of solid tumors. However, its use was limited due its dose-limiting toxicity to the kidney. Currently, there are no therapies available to treat or prevent cisplatin nephrotoxicity. Honey is a naturally occurring complex liquid and widely used in traditional Ayurvedic medicine to treat many illnesses. However, its effect on cisplatin nephrotoxicity is unknown. To determine the role of honey in cisplatin nephrotoxicity, animals were pretreated orally for a week and then cisplatin was administered. Honey feeding was continued for another 3 days. Our results show that animals with cisplatin-induced kidney dysfunction, as determined by increased serum creatinine, which received honey feeding had less kidney dysfunction. Improved kidney function was associated with better preservation of kidney morphology in honey-treated group as compared to the cisplatin alone-treated group. Interestingly, honey feeding significantly reduced cisplatin-induced tubular epithelial cell death, immune infiltration into the kidney as well as cytokine and chemokine expression and excretion as compared to cisplatin treated animals. Western blot analysis shows that cisplatin-induced increase in phosphorylation of NFkB was completely suppressed with honey feeding. In conclusion, honey feeding protects the kidney against cisplatin nephrotoxicity through suppression of inflammation and NFkB activation.
顺铂是一种高效的化疗药物，用于治疗多种实体瘤。然而，由于其对肾脏的剂量限制性毒性，其应用受到限制。目前，尚无治疗或预防顺铂肾毒性的疗法。蜂蜜是一种天然存在的复合液体，在传统阿育吠陀医学中广泛用于治疗多种疾病。然而，其对顺铂肾毒性的影响尚不清楚。为了确定蜂蜜在顺铂肾毒性中的作用，对动物进行了为期一周的口服预处理，然后给予顺铂。蜂蜜喂养持续另外3天。我们的结果表明，通过血清肌酐升高确定为顺铂诱导的肾功能不全的动物，接受蜂蜜喂养后肾功能不全较轻。与单独使用顺铂治疗的组相比，蜂蜜治疗组肾功能的改善与肾脏形态的更好保存有关。有趣的是，与顺铂治疗的动物相比，蜂蜜喂养显著减少了顺铂诱导的肾小管上皮细胞死亡、肾脏的免疫浸润以及细胞因子和趋化因子的表达和排泄。蛋白质印迹分析表明，蜂蜜喂养完全抑制了顺铂诱导的NFkB磷酸化增加。总之，蜂蜜喂养通过抑制炎症和NFkB激活来保护肾脏免受顺铂肾毒性的影响。
