Xu Hong-Xing, Hong Yue, Zhang Min-Zhu, Wang Yong-Liang, Liu Shu-Sheng, Wang Xiao-Wei
Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
Sci Rep. 2015 Jun 4;5:10774. doi: 10.1038/srep10774.
The whitefly Bemisia tabaci contains more than 35 cryptic species. The higher adaptability of Middle East-Asia Minor 1 (MEAM1) cryptic species has been recognized as one important factor for its invasion and displacement of other indigenous species worldwide. Here we compared the performance of the invasive MEAM1 and the indigenous Asia II 3 whitefly species following host plant transfer from a suitable host (cotton) to an unsuitable host (tobacco) and analyzed their transcriptional responses. After transfer to tobacco for 24 h, MEAM1 performed much better than Asia II 3. Transcriptional analysis showed that the patterns of gene regulation were very different with most of the genes up-regulated in MEAM1 but down-regulated in Asia II 3. Whereas carbohydrate and energy metabolisms were repressed in Asia II 3, the gene expression and protein metabolisms were activated in MEAM1. Compared to the constitutive high expression of detoxification genes in MEAM1, most of the detoxification genes were down-regulated in Asia II 3. Enzymatic activities of P450, GST and esterase further verified that the detoxification of MEAM1 was much higher than that of Asia II 3. These results reveal obvious differences in responses of MEAM1 and Asia II 3 to host transfer.
烟粉虱包含35种以上的隐种。中东-小亚细亚1型（MEAM1）隐种较高的适应性被认为是其在全球范围内入侵并取代其他本地物种的一个重要因素。在此，我们比较了入侵性的MEAM1和本地的亚洲II 3型粉虱在寄主植物从适宜寄主（棉花）转移到不适宜寄主（烟草）后的表现，并分析了它们的转录反应。转移到烟草上24小时后，MEAM1的表现远优于亚洲II 3型。转录分析表明，基因调控模式差异很大，MEAM1中的大多数基因上调，而亚洲II 3型中的大多数基因下调。亚洲II 3型中的碳水化合物和能量代谢受到抑制，而MEAM1中的基因表达和蛋白质代谢被激活。与MEAM1中解毒基因的组成型高表达相比，亚洲II 3型中的大多数解毒基因下调。细胞色素P450、谷胱甘肽S-转移酶和酯酶的酶活性进一步证实MEAM1的解毒能力远高于亚洲II 3型。这些结果揭示了MEAM1和亚洲II 3型对寄主转移反应的明显差异。
