Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci. 2020 Nov 11;21(22):8492. doi: 10.3390/ijms21228492.
(Gennadius) (Hemiptera: Aleyrodidae) is an important agricultural pest worldwide. Uridine diphosphate (UDP)-glucuronosyltransferases (UGTs) are one of the largest and most ubiquitous groups of proteins. Because of their role in detoxification, insect UGTs are attracting increasing attention. In this study, we identified and analyzed UGT genes in MEAM1 to investigate their potential roles in host adaptation and reproductive capacity. Based on phylogenetic and structural analyses, we identified 76 UGT genes in the MEAM1 genome. RNA-seq and real-time quantitative PCR (RT-qPCR) revealed differential expression patterns of these genes at different developmental stages and in association with four host plants (cabbage, cucumber, cotton and tomato). RNA interference results of selected UGTs showed that, when , , and were respectively silenced by feeding on dsRNA, the fecundity of MEAM1 was reduced, suggesting that the expressions of these three UGT genes in this species may be associated with host-related fecundity. Together, our results provide detailed UGTs data in and help guide future studies on the mechanisms of host adaptation by .
(Gennadius) （半翅目：粉虱科）是一种在世界范围内重要的农业害虫。尿苷二磷酸（UDP）-葡糖醛酸基转移酶（UGTs）是最大和最普遍的蛋白质之一。由于其在解毒中的作用，昆虫 UGTs 引起了越来越多的关注。在这项研究中，我们鉴定和分析了 MEAM1 中的 UGT 基因，以研究它们在宿主适应和繁殖能力方面的潜在作用。基于系统发育和结构分析，我们在 MEAM1 基因组中鉴定了 76 个 UGT 基因。RNA-seq 和实时定量 PCR（RT-qPCR）揭示了这些基因在不同发育阶段和与四种宿主植物（白菜、黄瓜、棉花和番茄）的相关性中的差异表达模式。对选定的 UGTs 的 RNA 干扰结果表明，当分别通过喂食 dsRNA 沉默 、 和 时，MEAM1 的繁殖力降低，这表明该物种中这三个 UGT 基因的表达可能与与宿主相关的繁殖力有关。总之，我们的结果提供了 MEAM1 中详细的 UGTs 数据，并有助于指导未来关于宿主适应机制的研究。
