a Stem Cell Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
b Student Research Committee, Tabriz University of Medical Sciences , Tabriz , Iran.
Artif Cells Nanomed Biotechnol. 2016 Aug;44(5):1303-10. doi: 10.3109/21691401.2015.1029623. Epub 2015 Jun 4.
Umbilical cord blood expresses cluster of differentiation (CD) 26, a fraction of CD34 + cells, negatively regulating in vivo homing and engraftment of hematopoietic stem cells. CD26 is highly expressed in various cells such as HSCs, immune cells, fibroblasts, and epithelial cells. It has been shown that inhibition of the CD26 on CD34 + cells improve the efficiency of transplantation of hematopoietic stem and progenitor cells. This study aimed to investigate the effect of key immune cell cytokines on CD26 expansion.
Cord blood mononuclear cells were cultured for 21 days using the stem cell factor, fetal liver tyrosine kinase 3 (Flt3) ligand (FL), interleukin (IL) 2, IL7, and IL15. Harvested cells were analyzed by flow cytometry at distinct time points.
Our results showed that utilization of IL7 significantly improved the expression of total CD26 + cells (8.6-fold higher). When either IL2 or IL15 were added to the culture, the expression also improved 2.5-fold. The IL2 and IL7 showed significant effect on the expansion of both the CD26 + and CD26 fractions of the CD34 + cells. However, the effects of IL15 on CD26 + and CD26 -expansion were similar.
Taken together, our data suggested that using IL7 causes higher proliferation of CD26 cells in comparison to that seen under other culture conditions.
脐带血表达分化群（CD）26，这是 CD34 + 细胞的一部分，负向调节造血干细胞体内归巢和植入。CD26 在各种细胞中高度表达，如造血干细胞、免疫细胞、成纤维细胞和上皮细胞。已经表明，抑制 CD34 + 细胞上的 CD26 可以提高造血干细胞和祖细胞移植的效率。本研究旨在探讨关键免疫细胞细胞因子对 CD26 扩增的影响。
使用干细胞因子、胎肝酪氨酸激酶 3（Flt3）配体（FL）、白细胞介素（IL）2、IL7 和 IL15 将脐带血单核细胞培养 21 天。在不同时间点通过流式细胞术分析收获的细胞。
我们的结果表明，使用 IL7 可显著提高总 CD26 + 细胞的表达（高 8.6 倍）。当向培养物中添加 IL2 或 IL15 时，表达也提高了 2.5 倍。IL2 和 IL7 对 CD34 + 细胞的 CD26 + 和 CD26 部分的扩增均有显著影响。然而，IL15 对 CD26 + 和 CD26 -扩增的影响相似。
综上所述，我们的数据表明，与其他培养条件相比，使用 IL7 可导致 CD26 细胞的增殖更高。
