Aliyari Z, Alami F, Mostafavi T, Taiefi Nasrabadi H, Soleimanirad J, Nozad Charoudeh H
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Iran J Ped Hematol Oncol. 2015;5(3):155-60. Epub 2015 Jul 20.
B cells play an important role in the immune system due to production of the immunoglobulin and secreting several cytokines. It has been shown that B cells produced successfully in the presence of stem cell factor (SCF) and Flt3 ligand (Flt3L). IL2, IL7, and IL15 cytokines as the γc-common family have an essential role in lymphopoiesis. Common γ chain cytokines may support either synergistically or in an additive manner to B cell proliferative activity. Thus, the present study focused specifically on IL2, IL7, and IL15 cytokines that may play a critical role in B cell proliferation in cord blood. In this study, we evaluated the generation of B cells from CD34+/- cord blood mononuclear cells by using IL-2, IL-7, and IL-15.
Cord blood mononuclear cells were cultured for 21 days in presence of different combination of IL-2, IL-7, and IL-15.Harvested cells were analyzed by flow cytometry at days 0, 7, 14 and, 21.
Present study showed that B cell differentiation from CD34+ cord blood mononuclear cells was increased by using IL-2 and IL-7 at different time points; however, IL7 was more effective (P value < 0.0001). In contrast, IL-15 didn't increase significantly B cell expansion from CD34+ cord blood mononuclear cell.
These findings showed that IL-2 and IL-7 significantly increased B cell generation from cord blood CD34+ cells; probably this cytokines may be used in ex vivo generation of B cells from cord blood mononuclear cells.
B细胞由于能产生免疫球蛋白并分泌多种细胞因子，在免疫系统中发挥着重要作用。研究表明，在干细胞因子（SCF）和Flt3配体（Flt3L）存在的情况下，B细胞能够成功产生。IL2、IL7和IL15细胞因子作为γc共同家族，在淋巴细胞生成中起着至关重要的作用。共同γ链细胞因子可能以协同或相加的方式支持B细胞的增殖活性。因此，本研究特别关注可能在脐带血B细胞增殖中起关键作用的IL2、IL7和IL15细胞因子。在本研究中，我们评估了使用IL-2、IL-7和IL-15从CD34+/-脐带血单个核细胞中生成B细胞的情况。
将脐带血单个核细胞在IL-2、IL-7和IL-15的不同组合存在的情况下培养21天。在第0、7、14和21天通过流式细胞术分析收获的细胞。
本研究表明，在不同时间点使用IL-2和IL-7可增加CD34+脐带血单个核细胞向B细胞的分化；然而，IL7更有效（P值<0.0001）。相比之下，IL-15并没有显著增加CD34+脐带血单个核细胞的B细胞扩增。
这些发现表明，IL-2和IL-7可显著增加脐带血CD34+细胞产生B细胞；可能这些细胞因子可用于从脐带血单个核细胞体外生成B细胞。
