Firat U, Senol S, Gelincik I, Kapan M, Tokgoz O, Tekin R, Evliyaoglu O, Onder A, Alp H
Department of Pathology, Dicle University, School of Medicine, Diyarbakir, Turkey.
Eur Rev Med Pharmacol Sci. 2015 May;19(10):1907-14.
Intestinal obstruction (IO) is a disease which generates approximately 20% of emergency surgery and tends to with high mortality. Prevention of oxidative stress, bacterial translocation and tissue damage caused by IO is an important medical issue. Caffeic acid phenethyl ester (CAPE) is an anti-inflammatory, antioxidant, anti-bacterial and immunomodulatory agent. In this experimental study, we aimed to investigate the effects of CAPE on bacterial translocation, inflammatory response, oxidative stress and tissue injury caused by intestinal obstruction in a rat model.
Breafly, thirty Wistar albino rats divided into three groups as Sham (n=10), IO (n=10) and IO + CAPE (10 µmol/kg day, intraperitoneal) (n=10). The tissues from the study groups were examined biochemically, microbiologically and histopathologically.
In CAPE treated group, decreased serum levels of proinflammatory cytokines (TNF-α, IL-6, IL-1β) and CRP (p < 0.05), additionally increased serum levels of antioxidant parameters (PONS, TAS) (p < 0.05), were observed after IO. Microbiologically, the rates of positive cultures of the lymph node, spleen, liver and blood were significantly decreased in CAPE treated group compared to the IO group. Also histopathological examination showed that the intestinal mucosal injury score and hepatic portal inflammation score were significantly decreased in the CAPE treated group (p < 0.05).
It is suggested that intraperitoneal administration of CAPE might has potential antibacterial, anti-inflammatory, antioxidant and immunomodulatory effects in IO. So, further studies on IO are needed to evaluate exact antibacterial, antiinflammatory, antioxidant and immunomodulatory effects of CAPE.
肠梗阻（IO）是一种约占急诊手术20%且死亡率较高的疾病。预防肠梗阻引起的氧化应激、细菌易位和组织损伤是一个重要的医学问题。咖啡酸苯乙酯（CAPE）是一种抗炎、抗氧化、抗菌和免疫调节药物。在本实验研究中，我们旨在探讨CAPE对大鼠肠梗阻模型中细菌易位、炎症反应、氧化应激和组织损伤的影响。
简而言之，30只Wistar白化大鼠分为三组，即假手术组（n = 10）、肠梗阻组（n = 10）和肠梗阻 + CAPE组（10 μmol/kg/天，腹腔注射）（n = 10）。对研究组的组织进行生化、微生物学和组织病理学检查。
在CAPE治疗组中，肠梗阻后观察到促炎细胞因子（TNF-α、IL-6、IL-1β）和CRP的血清水平降低（p < 0.05），此外抗氧化参数（PONS、TAS）的血清水平升高（p < 0.05）。微生物学检查显示，与肠梗阻组相比，CAPE治疗组淋巴结、脾脏、肝脏和血液的阳性培养率显著降低。组织病理学检查还显示，CAPE治疗组的肠黏膜损伤评分和肝门炎症评分显著降低（p < 0.05）。
提示腹腔注射CAPE可能对肠梗阻具有潜在的抗菌、抗炎、抗氧化和免疫调节作用。因此，需要对肠梗阻进行进一步研究，以评估CAPE确切的抗菌、抗炎、抗氧化和免疫调节作用。
