The Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin 4, Ireland.
Virology. 2012 May 25;427(1):51-9. doi: 10.1016/j.virol.2012.01.030. Epub 2012 Feb 27.
Recombination plays an important role in the evolutionary history of Hepatitis B virus (HBV). We performed a phylogenetic analysis of 3403 full-length HBV genome sequences isolated from humans to define the genotype. The genome sequences were divided into 13 sub-datasets, each approximately 250 bp in length. Genotype designations obtained from the sub-datasets that differed from the genotype defined by the whole genome were assigned as putative recombinants. Our results showed that 3379 out of 3403 sequences belonged to the previously described and putative genotypes A to J respectively, with 315 sequences defined in this analysis. The remaining 24 viruses had sequence divergence of less than 8% with both genotypes B and C and were provisionally assigned genotype "BC". 1047 out of 3403 sequences were identified to be putative recombinants, of which 72 were identified to be novel recombinants. Notably, all viruses of the herein described genotype "BC" were identified to be B/C recombinants.
重组在乙型肝炎病毒（HBV）的进化史中起着重要作用。我们对从人类中分离出的 3403 个全长 HBV 基因组序列进行了系统发育分析，以确定基因型。基因组序列分为 13 个子数据集，每个数据集的长度约为 250bp。与整个基因组定义的基因型不同的子数据集获得的基因型指定被指定为可能的重组体。我们的结果表明，3403 个序列中有 3379 个分别属于先前描述的和假定的基因型 A 至 J，其中 315 个序列在本次分析中定义。其余 24 种病毒与基因型 B 和 C 的序列差异小于 8%，暂时被分配为基因型“BC”。在 3403 个序列中，有 1047 个被确定为可能的重组体，其中 72 个被确定为新的重组体。值得注意的是，在此描述的基因型“BC”的所有病毒都被确定为 B/C 重组体。
