Hovik R, Osmundsen H
Department of Physiology and Biochemistry, Dental School, University of Oslo, Norway.
Biochem J. 1989 Oct 1;263(1):297-9. doi: 10.1042/bj2630297.
A direct-reading spectrophotometric assay for acyl-CoA oxidase activity is described. The assay is based on the strong absorption at 300 nm of deca-2-trans,4-cis-dienoyl-CoA, the product of oxidation of dec-4-cis-enoyl-CoA. By use of this assay, acetyl-CoA, CoA and FMN were found to be inhibitors of acyl-CoA oxidase, but with distinctly different kinetic characteristics.
本文描述了一种用于检测酰基辅酶A氧化酶活性的直接读数分光光度法。该检测方法基于癸-4-顺烯酰辅酶A氧化产物癸-2-反,4-顺二烯酰辅酶A在300nm处有强烈吸收。使用该检测方法发现,乙酰辅酶A、辅酶A和黄素单核苷酸是酰基辅酶A氧化酶的抑制剂,但具有明显不同的动力学特征。