Schremmer S D, Waser M R, Kohn M C, Garfinkel D
Comput Biomed Res. 1984 Jun;17(3):289-301. doi: 10.1016/s0010-4809(84)80020-8.
A computer program, PENNZYME (M. C. Kohn, L. E. Menten, and D. Garfinkel, Comput. Biomed. Res. 12, 461, 1979), that fits trial rate laws to enzyme and transport steady-state kinetic data by nonlinear regression has been enhanced. The new version includes a set of mnemonic commands which can be displayed in a menu at the user's option. Graphic displays of measured data and computed curves, as well as deviations between them (residual plots) have been added to aid the user in determining if a model is free of bias or systematic error. Other features include the ability to display the variance-covariance matrix of the parameters, to hold individual parameters constant during an optimization, and to optimize error models for the experimental data by the extended-least-squares technique.
一款名为PENNZYME的计算机程序（M. C. 科恩、L. E. 门滕和D. 加芬克尔，《计算机与生物医学研究》12卷，第461页，1979年）通过非线性回归将试验速率定律与酶和转运稳态动力学数据进行拟合，该程序得到了改进。新版本包括一组助记命令，用户可选择在菜单中显示这些命令。增加了测量数据和计算曲线的图形显示，以及它们之间的偏差（残差图），以帮助用户确定模型是否无偏差或系统误差。其他功能包括显示参数的方差 - 协方差矩阵的能力、在优化过程中使单个参数保持恒定的能力，以及通过扩展最小二乘法优化实验数据的误差模型的能力。
