Cennamo Nunzio, Pesavento Maria, Lunelli Lorenzo, Vanzetti Lia, Pederzolli Cecilia, Zeni Luigi, Pasquardini Laura
Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, Aversa, Italy.
Department of Chemistry, University of Pavia, Via Taramelli 12, Pavia, Italy.
Talanta. 2015 Aug 1;140:88-95. doi: 10.1016/j.talanta.2015.03.025. Epub 2015 Mar 21.
The introduction of new compact systems for sensitive, fast and simplified analysis is currently playing a substantial role in the development of point-of-care solutions aimed to assist both prognosis and diagnosis. Here we report a simple and low cost biosensor based on Surface Plasmon Resonance (SPR) taking advantage of a plastic optical fiber (POF) for the detection of Vascular endothelial growth factor (VEGF), selected as a circulating protein potentially associated with cancer. Our system is based onto two crucial aspects. By one hand, the functional layer which allows the transduction signal is based on DNA aptamers, short oligonucleotide sequences that bind to non-nucleic acid targets with high affinity and specificity. By the other hand, the light guiding structure is based on a POF with a planar gold layer as the sensing region, which is particularly suitable for bioreceptors implementation. The sensor revealed to be really useful in the interface characterization. The developed system is relatively easy to realize and could well address the development of a rapid, portable and low cost diagnostic platform, with a sensitivity in the nanomolar range.
新型紧凑系统的引入，用于灵敏、快速且简化的分析，目前在即时护理解决方案的开发中发挥着重要作用，这些解决方案旨在辅助预后和诊断。在此，我们报告一种基于表面等离子体共振（SPR）的简单且低成本的生物传感器，它利用塑料光纤（POF）来检测血管内皮生长因子（VEGF），VEGF被选为一种可能与癌症相关的循环蛋白。我们的系统基于两个关键方面。一方面，允许转导信号的功能层基于DNA适体，即与非核酸靶标具有高亲和力和特异性结合的短寡核苷酸序列。另一方面，光导结构基于具有平面金层作为传感区域的POF，这特别适合生物受体的实现。该传感器在界面表征方面显示出非常有用。所开发的系统相对容易实现，并且能够很好地满足快速、便携且低成本诊断平台的开发需求，灵敏度在纳摩尔范围内。
