Aray Ayda, Chiavaioli Francesco, Arjmand Mojtaba, Trono Cosimo, Tombelli Sara, Giannetti Ambra, Cennamo Nunzio, Soltanolkotabi Mahmood, Zeni Luigi, Baldini Francesco
Quantum Optics Group, Department of Physics, University of Isfahan, Hezar Jarib street, 98311, Isfahan, Islamic Republic of Iran.
Institute of Applied Physics "Nello Carrara", National Research Council, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Italy.
J Biophotonics. 2016 Oct;9(10):1077-1084. doi: 10.1002/jbio.201500315. Epub 2016 Apr 18.
A plastic optical fibre biosensor based on surface plasmon resonance for the detection of C-reactive protein (CRP) in serum is proposed. The biosensor was integrated into a home-made thermo-stabilized microfluidic system that allows avoiding any thermal and/or mechanical fluctuation and maintaining the best stable conditions during the measurements. A working range of 0.006-70 mg L and a limit of detection of 0.009 mg L were achieved. These results are among the best compared to other SPR-based biosensors for CRP detection, especially considering that they were achieved in a real and complex medium, i.e. serum. In addition, since the sensor performances satisfy those requested in physiologically-relevant clinical applications, the whole biosensing platform could well address high sensitive, easy to realize, real-time, label-free, portable and low cost diagnosis of CRP for future lab-on-a-chip applications. 3D sketch (left) of the thermo-stabilized home-made flow cell developed to house the SPR-based plastic optical fibre biosensor. Exemplary response curve (shift of the SPR wavelength versus time) of the proposed biosensor (right) for the detection of C-reactive protein in serum.
提出了一种基于表面等离子体共振的塑料光纤生物传感器，用于检测血清中的C反应蛋白（CRP）。该生物传感器集成到一个自制的热稳定微流控系统中，该系统可避免任何热波动和/或机械波动，并在测量过程中保持最佳稳定条件。实现了0.006 - 70 mg/L的工作范围和0.009 mg/L的检测限。与其他用于CRP检测的基于表面等离子体共振的生物传感器相比，这些结果是最好的之一，特别是考虑到它们是在真实复杂的介质即血清中获得的。此外，由于传感器性能满足生理相关临床应用的要求，整个生物传感平台可以很好地实现高灵敏度、易于实现、实时、无标记、便携且低成本的CRP诊断，以用于未来的芯片实验室应用。用于容纳基于表面等离子体共振的塑料光纤生物传感器的自制热稳定流通池的3D草图（左）。所提出的生物传感器用于检测血清中C反应蛋白的示例性响应曲线（表面等离子体共振波长随时间的变化）（右）。
