van der Wal Selina, Cornelissen Lisa, Behet Marije, Vaneker Michiel, Steegers Monique, Vissers Kris
Department of Anesthesiology, Pain and Palliative Medicine, Radboud University Nijmegen Medical Centre (RUNMC), 6525 GA, Nijmegen, the Netherlands.
Department of Microbiology RUNMC, 6525 GA, Nijmegen, the Netherlands.
Springerplus. 2015 May 15;4:225. doi: 10.1186/s40064-015-1009-4. eCollection 2015.
Neuropathic pain is defined as pain arising as a direct consequence of a lesion or disease affecting the somatosensory system and is common after surgery. Neuropathic pain can persist without an obvious injury. In this study we aim to validate a murine chronic constriction injury model as a model for neuropathic pain research and determine if silk or catgut ligatures induced most stable neuropathic pain behavior.
In this study mice underwent chronic constriction or sham surgery. Mice were tested on cutaneous hyperalgesia with the cumulative reaction time in the acetone test, on allodynia with the cumulative reaction time and number of lifts in the cold plate test and the maximal force before withdrawal in von Frey test.
In the acetone test neuropathic pain was seen in CCI mice, but not in sham mice. Hyperalgesia was present postoperatively in CCI mice compared with preoperatively. In the cold plate test cumulative reaction time and number of lifts were higher in the ipsilateral hind paw than in the contralateral hind paw and sham mice. Postoperative measurements were higher than preoperatively. In the von Frey test the postoperative measurements were lower in the ipsilateral hind paw than preoperatively, while the contralateral hind paw showed an increase in maximal force before withdrawal. The contralateral hind paw showed more difference with sham mice than the ipsilateral hind paw. Silk ligatures showed more stable neuropathic pain behavior. In the acetone test, the cold plate test and the von Frey test the mice scored higher on neuropathic pain having silk ligatures, compared with catgut ligatures.
In this study we validated a murine CCI model for neuropathic pain behavior. In the murine CCI model it appears that silk ligatures demonstrate more stable neuropathic pain behaviors than catgut ligatures in de CCI model.
神经性疼痛被定义为由影响躯体感觉系统的损伤或疾病直接导致的疼痛，在手术后很常见。神经性疼痛可在无明显损伤的情况下持续存在。在本研究中，我们旨在验证一种小鼠慢性压迫损伤模型作为神经性疼痛研究的模型，并确定丝线或肠线结扎是否能诱导出最稳定的神经性疼痛行为。
在本研究中，小鼠接受慢性压迫或假手术。通过丙酮试验中的累积反应时间来测试小鼠的皮肤痛觉过敏，通过冷板试验中的累积反应时间和抬起次数以及von Frey试验中的撤针前最大力来测试小鼠的异常性疼痛。
在丙酮试验中，CCI小鼠出现神经性疼痛，而假手术小鼠未出现。与术前相比，CCI小鼠术后出现痛觉过敏。在冷板试验中，同侧后爪的累积反应时间和抬起次数高于对侧后爪和假手术小鼠。术后测量值高于术前。在von Frey试验中，同侧后爪术后测量值低于术前，而对侧后爪撤针前最大力增加。对侧后爪与假手术小鼠的差异比同侧后爪更大。丝线结扎显示出更稳定的神经性疼痛行为。在丙酮试验、冷板试验和von Frey试验中，与肠线结扎相比，有丝线结扎的小鼠在神经性疼痛方面得分更高。
在本研究中，我们验证了一种用于神经性疼痛行为的小鼠CCI模型。在小鼠CCI模型中，在CCI模型中，丝线结扎似乎比肠线结扎表现出更稳定的神经性疼痛行为。
