Huang Xiaodong, Zheng Changkun, Wang Weiheng, Ye Xiaojian, Lin Chia-Ying, Wu Zenghui
Department of Orthopedics, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong, China.
Department of Orthopaedic Surgery, College of Medicine, University of Cincinnati, Cincinnati, OH, United States.
Front Neurosci. 2021 Mar 17;15:642436. doi: 10.3389/fnins.2021.642436. eCollection 2021.
To study the effect of intradiscal injection of simvastatin on discogenic pain in rats and its possible mechanism, 30 adult female rats were used in this experiment. Twenty rats were randomly divided into sham operation group (Control group), intervertebral disk degeneration group (DDD group), intervertebral disk degeneration + hydrogel group (DDD + GEL group), and intervertebral disk degeneration + simvastatin group (DDD + SIM group). The mechanical pain threshold and cold sensation in rats were measured. The contents of NF-kappa B1, RelA, GAP43, SP, CGRP, TRPM 8, IL-1β, and TNF-α in the intervertebral disk (IVD), the corresponding contents of dorsal root ganglion (DRG) and plantar skin GAP43 and TRPM 8 were quantitatively detected by PCR. The corresponding IVDs were stained to detect their degeneration. There was no significant difference in the mechanical pain threshold between the groups at each time point. From the first day to the 8th week after surgery, the cold-sensing response of the DDD group was significantly higher than that of the Control group ( < 0.05). At 7 and 8 weeks postoperatively, the cold-sensing response of the DDD + SIM group was significantly lower than that of the DDD + GEL group ( < 0.05). The levels of NF-κB1, RelA, GAP43, SP, CGRP, TRPM8, IL-1β, and TNF-α in the IVD of DDD + SIM group were significantly lower than those in DDD group ( < 0.05). The content of GAP43 and TRPM8 in rat plantar skin decreased significantly and TRPM8 in DRG decreased significantly ( < 0.05).
为研究椎间盘内注射辛伐他汀对大鼠椎间盘源性疼痛的影响及其可能机制，本实验选用30只成年雌性大鼠。将20只大鼠随机分为假手术组（对照组）、椎间盘退变组（DDD组）、椎间盘退变+水凝胶组（DDD+GEL组）和椎间盘退变+辛伐他汀组（DDD+SIM组）。测定大鼠的机械性疼痛阈值和冷觉。采用PCR定量检测椎间盘（IVD）中NF-κB1、RelA、GAP43、SP、CGRP、TRPM 8、IL-1β和TNF-α的含量，以及背根神经节（DRG）和足底皮肤中相应的GAP43和TRPM 8含量。对相应的IVD进行染色以检测其退变情况。各时间点各组间机械性疼痛阈值无显著差异。术后第1天至第8周，DDD组的冷觉反应显著高于对照组（P<0.05）。术后7周和8周，DDD+SIM组的冷觉反应显著低于DDD+GEL组（P<0.05）。DDD+SIM组IVD中NF-κB1、RelA、GAP43、SP、CGRP、TRPM8、IL-1β和TNF-α的水平显著低于DDD组（P<0.05）。大鼠足底皮肤中GAP43和TRPM8的含量显著降低，DRG中TRPM8的含量显著降低（P<0.05）。
